Document Detail


Redox status of the oviduct and CDC2 activity in 2-cell stage embryos in heat-stressed mice.
MedLine Citation:
PMID:  15028624     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mammalian preimplantation embryos are vulnerable to heat stress. However, the mechanisms by which maternal heat stress compromises embryonic development are unclear. We hypothesized that the loss of developmental competence in maternally heat-stressed embryos results from enhanced oxidative stress in the oviducts. In experiment 1, oviducts and zygotes were collected from mice that were heat-stressed at 35 degrees C and 60% relative humidity for 12 h on the day of pregnancy as well as from control mice. The zygotes were cultured for 84 h to assess their development, and the H(2)O(2) level, glutathione concentration, and free radical scavenging activity (FRSA) were measured in the oviduct. In experiment 2, zygotes were cultured for 22 h to reach the late G(2) phase in the 2-cell stage, and Cdc2 activity was assessed using immunoblotting. A high percentage (87.6%) of control embryos developed to morulae or blastocysts, whereas the majority (67.4%) of the heat-stressed group arrested at the 2-cell stage. Although heat stress did not alter the FRSA or glutathione concentration in the oviducts, the H(2)O(2) level (P < 0.01) and its ratio to the FRSA (P < 0.05) significantly increased in the heat-stressed group. The Cdc2 activation at the 2-cell stage, as shown by the ratio of the dephosphorylated form to the phosphorylated form, was evident in control embryos but absent in heat-stressed embryos, and the level was similar to that in embryos blocked at the 2-cell stage (positive control). These results indicate that maternal heat stress enhances oxidative stress in the oviducts and that loss of developmental competence in maternally heat-stressed embryos correlates with a defect in Cdc2 activity at the 2-cell stage.
Authors:
Manabu Ozawa; Takaya Matsuzuka; Miho Hirabayashi; Yukio Kanai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-03-17
Journal Detail:
Title:  Biology of reproduction     Volume:  71     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-06-22     Completed Date:  2005-01-05     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  291-6     Citation Subset:  IM    
Affiliation:
Institute of Agriculture and Forestry, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / metabolism*
Body Temperature
CDC2 Protein Kinase / metabolism*
Embryonic Development
Fallopian Tubes / enzymology,  metabolism*
Female
Heat Stress Disorders / metabolism*
Male
Mice
Mice, Inbred ICR
Oxidation-Reduction
Pregnancy
Pregnancy Complications / metabolism*
Rectum / physiopathology
Chemical
Reg. No./Substance:
EC 2.7.11.22/CDC2 Protein Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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