Document Detail

Redox status of the eye lens: a regional study.
MedLine Citation:
PMID:  15509888     Owner:  NLM     Status:  MEDLINE    
The aim of this work was to study the regional variation of some antioxidant systems in calf lens. Specific lens regions of nearly same age were obtained by a microsectioning technique, and the concentration of reduced and oxidized glutathione, protein sulfhydryl groups, and iron were measured in each lens region. The concentration of reduced glutathione, the major redox buffer in lens, exponentially decreased from the cortical regions to the nucleus. In contrast, the concentration of protein sulfhydryl groups gradually increased from the cortex toward the nucleus. The protein-bound disulfides remained constant throughout the lens. Iron was concentrated in the outer cortical region. The results show that the most dynamic redox-active zone in the lens is the subcapsular cortical region where the oxidant flux meets a highly reducing environment containing a potent redox catalyst.
Mariana Argirova; Mischa Kleine-Reidick; Winrich Breipohl
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell biochemistry and biophysics     Volume:  41     ISSN:  1559-0283     ISO Abbreviation:  Cell Biochem. Biophys.     Publication Date:  2004  
Date Detail:
Created Date:  2004-10-28     Completed Date:  2009-01-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9701934     Medline TA:  Cell Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  381-90     Citation Subset:  IM    
Department of Experimental Ophthalmology, University of Bonn, Abbéstr. 2, 53105 Bonn, Germany.
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MeSH Terms
Biophysics / methods
Cataract / metabolism
Cell Nucleus / metabolism
Disulfides / chemistry
Glutathione / metabolism*
Hydrogen Peroxide / chemistry
Iron / chemistry
Lens, Crystalline / metabolism*
Oxidative Stress
Oxygen / metabolism*
Proteins / chemistry
Reactive Oxygen Species
Reg. No./Substance:
0/Disulfides; 0/Proteins; 0/Reactive Oxygen Species; 70-18-8/Glutathione; 7439-89-6/Iron; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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