Document Detail


Redox processes in malaria and other parasitic diseases. Determination of intracellular glutathione.
MedLine Citation:
PMID:  7868369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of oxidative stress resulting from production of reactive oxygen species and/or from suppression of the cellular antioxidant capacity in parasitic infections is shortly reviewed. The experimental part of the paper deals with the glutathione (GSH)--glutathione reductase (GR) system, a cornerstone of intracellular antioxidant defence mechanisms. For studying this system in parasitic diseases such as malaria new or modified methods are required. Total glutathione comprising GSH and glutathione disulphide (GSSG) in blood samples was assayed as follows. One volume of blood (> or = 10 microliters) is mixed with two volumes of 5% sulphosalicylic acid; after centrifugation (5 min, 10000 g), 10 microliters of supernatant is taken for spectrophotometric analysis using the 5,5'-dithiobis(2-nitrobenzoate) (DTNB)-glutathione recycling assay. When compared with the original method, the procedure reported here is more sensitive, less time-consuming, avoids unfavourable pH-values and leads to a sample which when frozen is stable for months. In a pilot study, the method was applied to 14 patients suffering from malaria caused by Plasmodium falciparum. The concentrations of erythrocyte glutathione were significantly decreased in the patients (1.42 +/- 0.47 mM, mean +/- SD) when compared to age- and sex-matched controls (2.11 +/- 0.45 mM, P < 0.01). The findings are contrasted with P. falciparum cultures in vitro where glutathione levels are known to be elevated. Based on the characteristics of GR a concept of determining the redox state of single cells is introduced.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
K Becker; M Gui; A Traxler; C Kirsten; R H Schirmer
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Histochemistry     Volume:  102     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1995-03-30     Completed Date:  1995-03-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  389-95     Citation Subset:  IM    
Affiliation:
Institute of Biochemistry II, University of Heidelberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Child
Child, Preschool
Drug Stability
Female
Glutathione / analogs & derivatives,  blood*
Glutathione Disulfide
Glutathione Reductase / blood
Humans
Intracellular Fluid / metabolism
Malaria, Falciparum / blood*
Male
Oxidative Stress
Parasitic Diseases / metabolism*
Reproducibility of Results
Chemical
Reg. No./Substance:
27025-41-8/Glutathione Disulfide; 70-18-8/Glutathione; EC 1.8.1.7/Glutathione Reductase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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