Document Detail


Redox control of the catalytic cycle of flavocytochrome P-450 BM3.
MedLine Citation:
PMID:  9374858     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Flavocytochrome P-450 BM3 from Bacillus megaterium is a 119 kDa polypeptide whose heme and diflavin domains are fused to produce a catalytically self-sufficient fatty acid monooxygenase. Redox potentiometry studies have been performed with intact flavocytochrome P-450 BM3 and with its component heme, diflavin, FAD, and FMN domains. Results indicate that electron flow occurs from the NADPH donor through FAD, then FMN and on to the heme center where fatty acid substrate is bound and monooxygenation occurs. Prevention of futile cycling of electrons is avoided through an increase in redox potential of more than 100 mV caused by binding of fatty acids to the active site of P-450. Redox potentials are little altered for the component domains with respect to their values in the larger constructs, providing further evidence for the discrete domain organization of this flavocytochrome. The reduction potentials of the 4-electron reduced diflavin domain and 2-electron reduced FAD domain are considerably lower than those for the blue FAD semiquinone species observed during reductive titrations of these enzymes and that of the physiological electron donor (NADPH), indicating that the FAD hydroquinone is thermodynamically unfavorable and does not accumulate under turnover conditions. In contrast, the FMN hydroquinone is thermodynamically more favorable than the semiquinone.
Authors:
S N Daff; S K Chapman; K L Turner; R A Holt; S Govindaraj; T L Poulos; A W Munro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry     Volume:  36     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1997-12-08     Completed Date:  1997-12-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  13816-23     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of Edinburgh, U.K.
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MeSH Terms
Descriptor/Qualifier:
Bacillus megaterium / enzymology*
Bacterial Proteins*
Catalysis
Cytochrome P-450 Enzyme System / chemistry,  metabolism*
Flavin Mononucleotide / metabolism
Flavin-Adenine Dinucleotide / metabolism
Flavoproteins / chemistry,  metabolism*
Heme / metabolism
Mixed Function Oxygenases / chemistry,  metabolism*
NADPH-Ferrihemoprotein Reductase
Oxidation-Reduction
Potentiometry
Protein Structure, Tertiary
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Flavoproteins; 146-14-5/Flavin-Adenine Dinucleotide; 146-17-8/Flavin Mononucleotide; 14875-96-8/Heme; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Mixed Function Oxygenases; EC 1.6.2.4/NADPH-Ferrihemoprotein Reductase; EC 1.6.2.4/flavocytochrome P450 BM3 monoxygenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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