Document Detail

Redifferentiation of human gastric cancer cells induced by ascorbic acid and sodium selenite.
MedLine Citation:
PMID:  12500663     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To explore the effects and mechanisms of ascorbic acid (AA) and sodium selenite (SS) on growth inhibition and redifferentiation in human gastric cancer cells. METHODS: In the present study, trypan blue dye exclusion method was used to determine the cell growth curve and mitotic index, cell electrophoresis and colonogenic potential were used as the indexes of redifferentiation. In order to find out the mechanisms of redifferentiation, the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) were assayed, the content of malondialdehyde (MDA), reduced glutathione (GSH) and H2O2 were evaluated. RESULTS: After treatment with AA 3 mol/L + SS 2 mu mol/L, the growth rate and mitotic index of human gastric cancer cells (MGc-803) decreased remarkably. The indexes related with cell malignancy were alleviated. For example, cell surface charge was obviously decreased, the electrophoresis rate was dropped from 2.21 to 1.15 mu The indexes related with cell redifferentiation were promoted. For example, the colonogenic potential was decreased to 93.5%. These results indicated that redifferentiation of human gastric cancer cells was successfully induced by AA + SS. The activities of SOD and GPX were significantly higher, while the activity of CAT was slower in treated group than that in the control. The content of MDA was slightly decreased, GSH was sharply decreased, and H2O2 content was dramatically increased. CONCLUSION: These results indicated that combination of ascorbic acid and sodium selenite may induce the redifferentiation of human gastric cancer cells and inhibit cell growth by virtue of enhancing the activities of antioxidative enzymes and inducing the formation of H2O2, and altering the cell redox status. Combination of ascorbic acid and sodium selenite may be a potent anticancer agent for human gastric cancer.
Qiu-Sheng Zheng; Xi-Ling Sun; Chang-Hai Wang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedical and environmental sciences : BES     Volume:  15     ISSN:  0895-3988     ISO Abbreviation:  Biomed. Environ. Sci.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-12-25     Completed Date:  2003-04-01     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8909524     Medline TA:  Biomed Environ Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  223-32     Citation Subset:  IM    
Department of Biochemistry, Yantai University, Yantai 264005, Shandong, China.
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MeSH Terms
Antioxidants / pharmacology*
Ascorbic Acid / pharmacology*
Catalase / pharmacology
Cell Differentiation*
Glutathione Peroxidase / pharmacology
Mitotic Index
Sodium Selenite / pharmacology*
Stomach Neoplasms / pathology*
Superoxide Dismutase / pharmacology
Tumor Cells, Cultured
Reg. No./Substance:
0/Antioxidants; 10102-18-8/Sodium Selenite; 50-81-7/Ascorbic Acid; EC; EC Peroxidase; EC Dismutase

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