Document Detail


Redefining the relationship between sperm deoxyribonucleic acid fragmentation as measured by the sperm chromatin structure assay and outcomes of assisted reproductive techniques.
MedLine Citation:
PMID:  16084876     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To test the hypothesis that couples with sperm chromatin structure assay (SCSA) DNA fragmentation index (DFI) values >27% would not achieve pregnancy with assisted reproductive techniques (ART) and to investigate how DFI and high DNA stainability (HDS), as measured by the SCSA, affect fertilization, cleavage, implantation, and pregnancy rates in IVF cycles. DESIGN: Prospective clinical study. SETTING: Academic human reproduction laboratory. PATIENT(S): One hundred couples undergoing IVF with conventional insemination or intracytoplasmic sperm injection. INTERVENTION(S): Testing with SCSA was performed by SCSA Diagnostics (Brookings, South Dakota) on a semen aliquot taken from ejaculate used for ART. MAIN OUTCOME MEASURE(S): Relating total DFI and HDS to conventional semen parameters and cycle-specific outcomes after ART. RESULT(S): Nine of nineteen couples achieved clinical pregnancy when DFI was > or =27%, and 2 of 22 couples achieved clinical pregnancy when DFI was < or =9%. One of nine couples achieved clinical pregnancy with HDS >17%. The DFI was negatively correlated with sperm density (r = -0.23, P<.03) and motility (r = -0.55, P<.00), and HDS was negatively correlated with sperm density (r = -0.37, P<.00). CONCLUSION(S): Sperm chromatin structure assay failed to identify elevated DFI thresholds for negative pregnancy outcome after ART. Patients with low DFI (< or =9%) were least likely to become pregnant, which is also contradictory to SCSA marketing, which states that DFIs of < or =15% have excellent fertility potential. Patients with HDS > or =17% had low pregnancy rates, indicating decreased fertility potential, which deserves further investigation. Larger studies are necessary to confirm that low DFI is associated with decreased fertility and, if proved, might redefine the use of the SCSA in evaluating infertility.
Authors:
John F Payne; Douglas J Raburn; Grace M Couchman; Thomas M Price; Margaret G Jamison; David K Walmer
Related Documents :
7962436 - Pregnancy after intracytoplasmic sperm injection of metaphase ii oocytes with spermatoz...
3181496 - Successful pregnancies from men with retrograde ejaculation with the use of washed sper...
8034086 - The effect of antisperm autoantibodies in male or female partners undergoing in vitro f...
7128846 - Polyzoospermia: a definite pathologic entity.
2616516 - Bleeding in early pregnancy.
7418196 - Hair chromium at delivery in relation to age and number of pregnancies.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Fertility and sterility     Volume:  84     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-08-08     Completed Date:  2005-12-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  356-64     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Chromatin / physiology*
DNA Fragmentation / physiology*
Female
Humans
Linear Models
Male
Middle Aged
Pregnancy
Prospective Studies
Reproductive Techniques, Assisted*
Sperm Motility / physiology*
Spermatozoa / physiology*
Chemical
Reg. No./Substance:
0/Chromatin
Comments/Corrections
Comment In:
Fertil Steril. 2006 Mar;85(3):809; author reply 809-10   [PMID:  16500373 ]
Fertil Steril. 2006 Mar;85(3):810-1; author reply 811-2   [PMID:  16500374 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Association of abnormal morphology and altered gene expression in human preimplantation embryos.
Next Document:  Fluorescence in situ hybridization sperm analysis of six translocation carriers provides evidence of...