|Redefining the properties of an osmotic agent in an intestinal-specific preservation solution.|
|PMID: 21128319 Owner: NLM Status: MEDLINE|
|AIM: To investigate the effects of dextrans of various molecular weights (Mw) during a 12 h cold storage time-course on energetics, histology and mucosal infiltration of fluorescein isothiocyanate (FITC)-dextran.
METHODS: Rodent intestines were isolated and received a standard University of Wisconsin vascular flush followed by intraluminal administration of a nutrient-rich preservation solution containing dextrans of varying Mw: Group D1, 73 kdal; Group D2, 276 kdal; Group D3, 534 kdal; Group D4, 1185 kdal; Group D5, 2400 kdal.
RESULTS: Using FITC-labeled dextrans, fluorescent micrographs demonstrated varying degrees of mucosal infiltration; lower Mw (groups D1-D3: 73-534 kdal) dextrans penetrated the mucosa as early as 2 h, whereas the largest dextran (D5: 2400 kdal) remained captive within the lumen and exhibited no permeability even after 12 h. After 12 h, median injury grades ranged from 6.5 to 7.5 in groups D1-D4 (73-1185 kdal) representing injury of the regenerative cryptal regions and submucosa; this was in contrast to group D5 (2400 kdal) which exhibited villus denudation (with intact crypts) corresponding to a median injury grade of 4 (P < 0.05). Analysis of tissue energetics reflected a strong positive correlation between Mw and adenosine triphosphate (r(2) = 0.809), total adenylates (r(2) = 0.865) and energy charge (r(2) = 0.667).
CONCLUSION: Our data indicate that dextrans of Mw > 2400 kdal act as true impermeant agents during 12 h ischemic storage when incorporated into an intraluminal preservation solution.
|Kimberly Schlachter; Matthew S Kokotilo; Jodi Carter; Aducio Thiesen; Angela Ochs; Rachel G Khadaroo; Thomas A Churchill|
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|Type: Journal Article; Research Support, Non-U.S. Gov't|
|Title: World journal of gastroenterology : WJG Volume: 16 ISSN: 2219-2840 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2010 Dec|
|Created Date: 2010-12-03 Completed Date: 2011-03-21 Revised Date: 2014-05-20|
Medline Journal Info:
|Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China|
|Languages: eng Pagination: 5701-9 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
Adenosine Triphosphate / metabolism
Allopurinol / chemistry, pharmacology
Cold Ischemia* / adverse effects
Dextrans / chemistry, metabolism, pharmacology*
Fluorescein-5-isothiocyanate / analogs & derivatives, metabolism
Glutathione / chemistry, pharmacology
Insulin / chemistry, pharmacology
Intestinal Mucosa / drug effects*, metabolism, pathology, transplantation
Intestine, Small / drug effects*, metabolism, pathology, transplantation
Organ Preservation Solutions / chemistry, pharmacology*
Oxidative Stress / drug effects
Raffinose / chemistry, pharmacology
Reperfusion Injury / etiology, metabolism, pathology, prevention & control*
|//Canadian Institutes of Health Research|
|0/Insulin; 0/Organ Preservation Solutions; 0/University of Wisconsin-lactobionate solution; 0/fluorescein isothiocyanate dextran; 63CZ7GJN5I/Allopurinol; 8L70Q75FXE/Adenosine Triphosphate; GAN16C9B8O/Glutathione; I223NX31W9/Fluorescein-5-isothiocyanate; K3R6ZDH4DU/Dextrans; K72T3FS567/Adenosine; N5O3QU595M/Raffinose|
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