| Redefining the properties of an osmotic agent in an intestinal-specific preservation solution. | |
| | |
MedLine Citation:
|
PMID: 21128319 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
AIM: to investigate the effects of dextrans of various molecular weights (Mw) during a 12 h cold storage time-course on energetics, histology and mucosal infiltration of fluorescein isothiocyanate (FITC)-dextran. METHODS: rodent intestines were isolated and received a standard University of Wisconsin vascular flush followed by intraluminal administration of a nutrient-rich preservation solution containing dextrans of varying Mw: Group D1, 73 kdal; Group D2, 276 kdal; Group D3, 534 kdal; Group D4, 1185 kdal; Group D5, 2400 kdal. RESULTS: using FITC-labeled dextrans, fluorescent micrographs demonstrated varying degrees of mucosal infiltration; lower Mw (groups D1-D3: 73-534 kdal) dextrans penetrated the mucosa as early as 2 h, whereas the largest dextran (D5: 2400 kdal) remained captive within the lumen and exhibited no permeability even after 12 h. After 12 h, median injury grades ranged from 6.5 to 7.5 in groups D1-D4 (73-1185 kdal) representing injury of the regenerative cryptal regions and submucosa; this was in contrast to group D5 (2400 kdal) which exhibited villus denudation (with intact crypts) corresponding to a median injury grade of 4 (P < 0.05). Analysis of tissue energetics reflected a strong positive correlation between Mw and adenosine triphosphate (r(2) = 0.809), total adenylates (r(2) = 0.865) and energy charge (r(2) = 0.667). CONCLUSION: our data indicate that dextrans of Mw > 2400 kdal act as true impermeant agents during 12 h ischemic storage when incorporated into an intraluminal preservation solution. |
| | |
Authors:
|
Kimberly Schlachter; Matthew S Kokotilo; Jodi Carter; Aducio Thiesen; Angela Ochs; Rachel G Khadaroo; Thomas A Churchill |
Related Documents
:
|
21153029 - Cilostazol reduces the progression of carotid intima-media thickness without increasing... 21336439 - Early postoperative oral feeding impacts positively in patients undergoing colonic rese... 21180029 - Percuteneous mitral balloon valvuloplasty in patients with post surgical mitral resteno... 21161419 - Physician groups' use of data from patient experience surveys. 10836289 - Remission of severe tardive dyskinesia in a schizophrenic patient treated with the atyp... 23306419 - Revision total hip arthroplasty in younger patients: indications, reasons for failure, ... 22883359 - Effects of folk medicinal plant extract ankaferd blood stopper(®) in vital primary mol... 8989009 - Prevention of spinal anesthesia-induced hypotension in the elderly: comparison between ... 660789 - Myocardial reinfarction after anesthesia and surgery. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: World journal of gastroenterology : WJG Volume: 16 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-12-03 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
|
Languages: eng Pagination: 5701-9 Citation Subset: IM |
Affiliation:
|
Department of Surgery, University of Alberta, 2D2.28 Walter Mackenzie Center, Edmonton, Alberta, T6G 2B7, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Grant Support | |
ID/Acronym/Agency:
|
//Canadian Institutes of Health Research |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Insulin like growth factor-1 increases fatty liver preservation in IGL-1 solution.
Next Document: Role of simple biomarkers in predicting fibrosis progression in HCV infection.