Document Detail


Redefining the properties of an osmotic agent in an intestinal-specific preservation solution.
MedLine Citation:
PMID:  21128319     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate the effects of dextrans of various molecular weights (Mw) during a 12 h cold storage time-course on energetics, histology and mucosal infiltration of fluorescein isothiocyanate (FITC)-dextran.
METHODS: Rodent intestines were isolated and received a standard University of Wisconsin vascular flush followed by intraluminal administration of a nutrient-rich preservation solution containing dextrans of varying Mw: Group D1, 73 kdal; Group D2, 276 kdal; Group D3, 534 kdal; Group D4, 1185 kdal; Group D5, 2400 kdal.
RESULTS: Using FITC-labeled dextrans, fluorescent micrographs demonstrated varying degrees of mucosal infiltration; lower Mw (groups D1-D3: 73-534 kdal) dextrans penetrated the mucosa as early as 2 h, whereas the largest dextran (D5: 2400 kdal) remained captive within the lumen and exhibited no permeability even after 12 h. After 12 h, median injury grades ranged from 6.5 to 7.5 in groups D1-D4 (73-1185 kdal) representing injury of the regenerative cryptal regions and submucosa; this was in contrast to group D5 (2400 kdal) which exhibited villus denudation (with intact crypts) corresponding to a median injury grade of 4 (P < 0.05). Analysis of tissue energetics reflected a strong positive correlation between Mw and adenosine triphosphate (r(2) = 0.809), total adenylates (r(2) = 0.865) and energy charge (r(2) = 0.667).
CONCLUSION: Our data indicate that dextrans of Mw > 2400 kdal act as true impermeant agents during 12 h ischemic storage when incorporated into an intraluminal preservation solution.
Authors:
Kimberly Schlachter; Matthew S Kokotilo; Jodi Carter; Aducio Thiesen; Angela Ochs; Rachel G Khadaroo; Thomas A Churchill
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  16     ISSN:  2219-2840     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-03     Completed Date:  2011-03-21     Revised Date:  2014-05-20    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  5701-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine / chemistry,  pharmacology
Adenosine Triphosphate / metabolism
Allopurinol / chemistry,  pharmacology
Animals
Cold Ischemia* / adverse effects
Dextrans / chemistry,  metabolism,  pharmacology*
Energy Metabolism
Fluorescein-5-isothiocyanate / analogs & derivatives,  metabolism
Glutathione / chemistry,  pharmacology
Insulin / chemistry,  pharmacology
Intestinal Absorption
Intestinal Mucosa / drug effects*,  metabolism,  pathology,  transplantation
Intestine, Small / drug effects*,  metabolism,  pathology,  transplantation
Molecular Weight
Organ Preservation Solutions / chemistry,  pharmacology*
Osmosis
Oxidative Stress / drug effects
Permeability
Raffinose / chemistry,  pharmacology
Rats
Rats, Sprague-Dawley
Reperfusion Injury / etiology,  metabolism,  pathology,  prevention & control*
Time Factors
Grant Support
ID/Acronym/Agency:
//Canadian Institutes of Health Research
Chemical
Reg. No./Substance:
0/Insulin; 0/Organ Preservation Solutions; 0/University of Wisconsin-lactobionate solution; 0/fluorescein isothiocyanate dextran; 63CZ7GJN5I/Allopurinol; 8L70Q75FXE/Adenosine Triphosphate; GAN16C9B8O/Glutathione; I223NX31W9/Fluorescein-5-isothiocyanate; K3R6ZDH4DU/Dextrans; K72T3FS567/Adenosine; N5O3QU595M/Raffinose
Comments/Corrections

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