Document Detail

Red cell membrane lipid changes at 3,500 m and on return to sea level.
MedLine Citation:
PMID:  16351566     Owner:  NLM     Status:  MEDLINE    
Previous studies have shown that acute hypobaric hypoxia, obtained in a hypobaric chamber, and subsequent reoxygenation, give rise to modifications of the erythrocyte membrane lipid dynamics, resulting in an increased lateral diffusivity of the membrane lipids, and this was interpreted as the result of a modified lipid-protein interaction. The aim of the present study was to determine the effect of the reoxygenation condition in individuals after 3 days at an altitude of 3,500 m above sea level. Reoxygenation was a consequence of returning to sea level. Resting blood samples from both conditions were obtained, and erythrocytes were separated and immediately lysed for membrane isolation. We measured the bilayer polarity in membranes with Laurdan, a fluorescent probe. We also measured malondialdehyde in membrane lipids, an indicator of oxidative damage. We found a 12% (p = 0.016, n = 7) increase in the polarity of the membrane bilayer surface, and an increase of 70% (p = 0.005, n = 7) in the formation of malondialdehyde in the membrane after the reoxygenation condition. The membrane bilayer polarity increase is due to an oxidative modification of the phospholipid backbone after reoxygenation. People working and/or recreating at moderate altitude (3,500 m) may be at risk of erythrocyte membrane oxidative damage upon returning to sea level, and therefore a better understanding of the processes occurring upon reoxygenation may lead to proposed strategies to minimize this effect.
Gustavo Gonz?lez; Gloria Celed?n; Marcela Escobar; Carlos Sotomayor; Ver?nica Ferrer; Dixan Ben?tez; Claus Behn
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  High altitude medicine & biology     Volume:  6     ISSN:  1527-0297     ISO Abbreviation:  High Alt. Med. Biol.     Publication Date:  2005  
Date Detail:
Created Date:  2005-12-14     Completed Date:  2006-04-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901183     Medline TA:  High Alt Med Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  320-6     Citation Subset:  IM; S    
Instituto de Qu?mica, Facultad de Ciencias B?sicas y Matem?ticas, Pontificia Universidad Cat?lica de Valpara?so, Casilla 4059, Chile.
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MeSH Terms
Acute Disease
Altitude Sickness / blood*
Chromatography, High Pressure Liquid
Erythrocyte Membrane / metabolism*
Membrane Lipids / metabolism*
Oxidative Stress*
Reg. No./Substance:
0/Membrane Lipids

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