| Red blood cell volume can be independently determined in vitro using sheep and human red blood cells labeled at different densities of biotin. | |
| | |
MedLine Citation:
|
PMID: 19220818 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: The development of valid methods for repeatedly measuring red blood cell (RBC) volume (RCV) in the same individual would be useful in furthering understanding of the physiology and pathophysiology of the pregnant woman, fetus, and infant under a variety of conditions. STUDY DESIGN AND METHODS: Small volumes (5 to 100 mL) of either sheep or human blood were used to test the hypothesis that there is no significant difference in RCV and blood volume determined in vitro using as many as five populations of RBCs labeled at distinct biotin densities. By varying the mass of biotinylating reagent, the density of biotin on the surface of RBCs was incrementally increased to produce discrete populations as assessed by flow cytometric enumeration. Calculation of RCV for each biotin-labeled RBC population was based on the dilution principle. RESULTS: All biotin densities, except the most densely labeled, where variance was the greatest, accurately quantitated the in vitro blood volume to within 10 percent of the correct value. There was no bias of either overestimation or underestimation in the determination of the blood volume using either sheep or human RBCs. CONCLUSION: These in vitro results provide evidence that the multidensity biotin labeling method is sufficiently accurate to utilize in vivo for repeated determination of circulating RCV and blood volume. |
| | |
Authors:
|
Donald M Mock; Nell I Matthews; Ronald G Strauss; Leon F Burmeister; Robert Schmidt; John A Widness |
Related Documents
:
|
16965128 - Respiration-induced changes in tissue blood volume distal to occluded artery, measured ... 8431188 - Blood volume reduction counteracts fluid shifts in water immersion. 11039648 - Adaptations to a 7-day head-down bed rest with thigh cuffs. 6885578 - Two-sidearm tracheal cannula for respiratory airflow measurements in small animals. 6974458 - Bronchospasm during excretory urography: lack of specificity for the methylglucamine ca... 21941008 - Salivary cortisol, heart rate and blood lactate responses during elite downhill mountai... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-02-10 |
Journal Detail:
|
Title: Transfusion Volume: 49 ISSN: 1537-2995 ISO Abbreviation: Transfusion Publication Date: 2009 Jun |
Date Detail:
|
Created Date: 2009-07-29 Completed Date: 2009-08-11 Revised Date: 2010-12-03 |
Medline Journal Info:
|
Nlm Unique ID: 0417360 Medline TA: Transfusion Country: United States |
Other Details:
|
Languages: eng Pagination: 1178-85 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry & Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA. mockdonaldm@uams.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biotinylation* Erythrocyte Volume* Flow Cytometry Humans Sheep |
| Grant Support | |
ID/Acronym/Agency:
|
P01 HL046925/HL/NHLBI NIH HHS; P01 HL046925-13/HL/NHLBI NIH HHS; P01 HL046925-15/HL/NHLBI NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Risk of introduction of H5N1 HPAI from Europe to Spain by wild water birds in autumn.
Next Document: Critical evaluation of informed consent forms for adult and minor aged whole blood donation used by ...