Document Detail

Red blood cell delta15N: a novel biomarker of dietary eicosapentaenoic acid and docosahexaenoic acid intake.
MedLine Citation:
PMID:  19176727     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The long-chain omega-3 (n-3) fatty acids derived from fish, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are associated with a reduced risk of cardiovascular disease and other chronic diseases. Study of the associations between EPA and DHA intake and disease requires a valid biomarker of dietary intake; however, the direct measurement of tissue fatty acid concentrations is expensive and time consuming.
OBJECTIVE: Because the nitrogen stable isotope ratio ((15)N/(14)N, expressed as delta(15)N) is elevated in fish, we investigated whether delta(15)N is a valid alternative biomarker of EPA and DHA intake.
DESIGN: We examined the relation between red blood cell (RBC) delta(15)N and RBC EPA and DHA in a community-based sample of 496 Yup'ik Eskimos with widely varying intake of n-3 fatty acids. We also assessed the correlation between delta(15)N and dietary EPA and DHA intake based on 24-h dietary recalls and 3-d food records completed by a subset of 221 participants.
RESULTS: RBC delta(15)N was strongly correlated with RBC EPA and DHA (r = 0.83 and 0.75, respectively). These correlations differed only modestly by sex and age class. RBC delta(15)N also correlated with dietary EPA and DHA intake (r = 0.47 and 0.46, respectively) and did not differ by sex and age.
CONCLUSIONS: The results strongly support the validity of RBC delta(15)N as a biomarker of EPA and DHA intake. Because the analysis of RBC delta(15)N is rapid and inexpensive, this method could facilitate wide-scale assessment of EPA and DHA intake in clinical and epidemiologic studies.
Diane M O'Brien; Alan R Kristal; M Alyssa Jeannet; Michael J Wilkinson; Andrea Bersamin; Bret Luick
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-01-28
Journal Detail:
Title:  The American journal of clinical nutrition     Volume:  89     ISSN:  1938-3207     ISO Abbreviation:  Am. J. Clin. Nutr.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-02-23     Completed Date:  2009-03-18     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  0376027     Medline TA:  Am J Clin Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  913-9     Citation Subset:  AIM; IM    
Center for Alaska Native Health Research, Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, AK 99775-7000, USA.
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MeSH Terms
Age Factors
Biological Markers / blood
Cross-Sectional Studies
Dietary Fats / administration & dosage,  blood*
Docosahexaenoic Acids / administration & dosage,  blood*
Eicosapentaenoic Acid / administration & dosage,  blood*
Erythrocytes / metabolism*
Linear Models
Middle Aged
Nitrogen Isotopes / blood*
Sex Factors
Young Adult
Grant Support
P20 RR016430/RR/NCRR NIH HHS; P20 RR016430-01/RR/NCRR NIH HHS; P20 RR016466-066878/RR/NCRR NIH HHS; P20 RR16430/RR/NCRR NIH HHS; RR016466/RR/NCRR NIH HHS
Reg. No./Substance:
0/Biological Markers; 0/Dietary Fats; 0/Nitrogen Isotopes; 1553-41-9/Eicosapentaenoic Acid; 25167-62-8/Docosahexaenoic Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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