Document Detail


Red blood cell aggregation, aggregate strength and oxygen transport potential of blood are abnormal in both homozygous sickle cell anemia and sickle-hemoglobin C disease.
MedLine Citation:
PMID:  19644138     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Recent evidence suggests that red blood cell aggregation and the ratio of hematocrit to blood viscosity (HVR), an index of the oxygen transport potential of blood, might considerably modulate blood flow dynamics in the microcirculation. It thus seems likely that these factors could play a role in sickle cell disease. DESIGN AND METHODS: We compared red blood cell aggregation characteristics, blood viscosity and HVR at different shear rates between sickle cell anemia and sickle cell hemoglobin C disease (SCC) patients, sickle cell trait carriers (AS) and control individuals (AA). RESULTS: Blood viscosity determined at high shear rate was lower in sickle cell anemia (n=21) than in AA (n=52), AS (n=33) or SCC (n=21), and was markedly increased in both SCC and AS. Despite differences in blood viscosity, both sickle cell anemia and SCC had similar low HVR values compared to both AA and AS. Sickle cell anemia (n=21) and SCC (n=19) subjects had a lower red blood cell aggregation index and longer time for red blood cell aggregates formation than AA (n=16) and AS (n=15), and a 2 to 3 fold greater shear rate required to disperse red blood cell aggregates. CONCLUSIONS: The low HVR levels found in sickle cell anemia and SCC indicates a comparable low oxygen transport potential of blood in both genotypes. Red blood cell aggregation properties are likely to be involved in the pathophysiology of sickle cell disease: the increased shear forces needed to disperse red blood cell aggregates may disturb blood flow, especially at the microcirculatory level, since red blood cell are only able to pass through narrow capillaries as single cells rather than as aggregates.
Authors:
Julien Tripette; Tamas Alexy; Marie-Dominique Hardy-Dessources; Daniele Mougenel; Eric Beltan; Tawfik Chalabi; Roger Chout; Maryse Etienne-Julan; Olivier Hue; Herbert J Meiselman; Philippe Connes
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Haematologica     Volume:  94     ISSN:  1592-8721     ISO Abbreviation:  Haematologica     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-31     Completed Date:  2009-12-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417435     Medline TA:  Haematologica     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  1060-5     Citation Subset:  IM    
Affiliation:
Laboratoire ACTES, EA 3596, Département de Physiologie, Université des Antilles et de la Guyane, Campus de Fouillole, Pointe-à-Pitre, Guadeloupe, French West Indies.
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MeSH Terms
Descriptor/Qualifier:
Anemia, Sickle Cell / blood*,  genetics
Biological Transport
Blood Viscosity
Erythrocyte Aggregation*
Erythrocyte Deformability
Erythrocytes / metabolism
Fibrinogen / metabolism
Hematocrit
Hemoglobin C / metabolism
Hemoglobin C Disease / blood*,  genetics
Hemoglobin, Sickle / metabolism
Hemorheology
Homozygote
Humans
Oxygen / metabolism*
Chemical
Reg. No./Substance:
0/Hemoglobin, Sickle; 7782-44-7/Oxygen; 9001-32-5/Fibrinogen; 9008-00-8/Hemoglobin C
Comments/Corrections

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