| Red blood cell (RBC) volume can be independently determined in vivo in humans using RBCs labeled at different densities of biotin. | |
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MedLine Citation:
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PMID: 20630041 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Anemia is a serious problem in critically ill neonates. To investigate the pathophysiology of anemia and responses to red blood cell (RBC) transfusions and erythropoietin therapy, repeated measurement of red blood cell volume (RCV) and blood volume is useful. To extend our previous sheep study in which RBCs were labeled at four different biotin densities, we assessed the validity of this multidensity method for in vivo measurement of circulating RCV in humans. STUDY DESIGN AND METHODS: In eight healthy adults, autologous RBCs were biotinylated at each of four biotin densities (6, 18, 54, and 162 µg biotinylation reagent/mL RBC), mixed, and infused intravenously; blood was sampled at 10, 20, and 60 minutes. At each time, RCV was calculated from dilution of individual RBC populations enumerated by flow cytometry. RCV measurements from the population of RBCs biotinylated at 6 µg/mL were chosen as the reference values because this density had been previously validated against the 51Cr method in vitro and in vivo in humans. RESULTS: Values for RCVs were not significantly different among the four densities of biotinylated RBCs at any of the three time points and did not change over 60 minutes. CONCLUSIONS: These studies provide evidence that four densities of biotinylated RBCs can be used in vivo for simultaneous, independent, accurate measurements of RCV in humans. We speculate that this method will also be useful for repeated measurement of RCV and blood volume in infants and other patient populations in whom radioactive labels should be avoided. |
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Authors:
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Donald M Mock; Nell I Matthews; Shan Zhu; Leon F Burmeister; M Bridget Zimmerman; Ronald G Strauss; Robert L Schmidt; Demet Nalbant; Gretchen A Cress; John A Widness |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Transfusion Volume: 51 ISSN: 1537-2995 ISO Abbreviation: Transfusion Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-11 Completed Date: 2011-02-08 Revised Date: 2012-01-04 |
Medline Journal Info:
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Nlm Unique ID: 0417360 Medline TA: Transfusion Country: United States |
Other Details:
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Languages: eng Pagination: 148-57 Citation Subset: IM |
Copyright Information:
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© 2010 American Association of Blood Banks. |
Affiliation:
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Department of Biochemistry & Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA. mockdonaldm@uams.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Biotin / metabolism* Erythrocyte Volume* Erythrocytes / cytology*, metabolism* Humans Middle Aged Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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P01 HL046925/HL/NHLBI NIH HHS; P01 HL046925-15/HL/NHLBI NIH HHS; UL1RR024979/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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58-85-5/Biotin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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