| Red blood cell (RBC) survival determined in humans using RBCs labeled at multiple biotin densities. | |
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MedLine Citation:
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PMID: 21062290 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Safe, accurate methods permitting simultaneous and/or repeated measurement of red blood cell (RBC) survival (RCS) are important to investigate pathophysiology and therapy of anemia. Methods using chromium 51 ((51) Cr)-labeled RBCs are unacceptable for infants, children, and pregnant women. We report RCS measured in vivo using RBCs labeled with several densities of biotin (BioRBCs). STUDY DESIGN AND METHODS: Aliquots of autologous RBCs from eight healthy adult subjects were labeled separately at four discrete biotin densities, mixed, and infused. The proportion of each population of BioRBCs circulating was determined serially by flow cytometry over 20 weeks. For each population, RCS was assessed by the following: 1) posttransfusion BioRBC recovery at 24 hours (PTR(24) ); 2) time to decrease to 50% of the enrichment at 24 hours (T(50) ); and 3) mean potential lifespan (MPL). RESULTS: Among the four BioRBC densities, no significant differences in PTR(24) were observed. T(50) and MPL were similar for the two lowest BioRBC densities. In contrast, the two highest BioRBC densities demonstrated progressively decreased T(50) and MPL. CONCLUSIONS: RBCs labeled at four biotin densities can be used to independently and accurately measure PTR(24 ) and two lowest biotin densities can accurately quantitate long-term RCS. This method provides a tool for investigating anemia in infants, fetuses, and pregnant women with the following advantages over the standard (51) Cr method: 1) study subjects are not exposed to radiation; 2) small blood volumes (e.g., 20 µL) are required; and 3) multiple independent RCS measurements can be made simultaneously in the same individual. |
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Authors:
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Donald M Mock; Nell I Matthews; Shan Zhu; Ronald G Strauss; Robert L Schmidt; Demet Nalbant; Gretchen A Cress; John A Widness |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-11-09 |
Journal Detail:
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Title: Transfusion Volume: 51 ISSN: 1537-2995 ISO Abbreviation: Transfusion Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-05-06 Completed Date: 2011-07-12 Revised Date: 2012-05-02 |
Medline Journal Info:
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Nlm Unique ID: 0417360 Medline TA: Transfusion Country: United States |
Other Details:
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Languages: eng Pagination: 1047-57 Citation Subset: IM |
Copyright Information:
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© 2010 American Association of Blood Banks. |
Affiliation:
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Department of Biochemistry & Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA. mockdonaldm@uams.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antibodies / blood Biotin / immunology, metabolism* Biotinylation / methods* Cell Survival / physiology Erythrocyte Transfusion / standards* Erythrocytes / cytology*, immunology, metabolism Female Flow Cytometry / methods*, standards Haptoglobins / metabolism Hemolysis Humans Male Middle Aged Reference Standards Staining and Labeling / methods Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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P01 HL046925/HL/NHLBI NIH HHS; P01 HL046925-15/HL/NHLBI NIH HHS; UL1RR024979/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Haptoglobins; 58-85-5/Biotin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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