| Red Light, phot1 and JAC1 Modulate phot2-dependent Reorganization of Chloroplast Actin Filaments and Chloroplast Avoidance Movement. | |
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MedLine Citation:
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PMID: 21737483 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The phototropin-dependent intracellular relocation of chloroplasts is a ubiquitous phenomenon in plants. We have previously revealed the involvement of a short cp-actin (chloroplast actin) filament-based mechanism in this movement. Here, the reorganization of cp-actin filaments during the avoidance movement of chloroplasts was analyzed in higher time resolution under blue GFP excitation light in an actin filament-visualized line of Arabidopsis thaliana. Under standard background red light of 89 μmol m(-2) s(-1), cp-actin filaments transiently disappeared at approximately 30 sec and reappeared in a biased configuration on chloroplasts approximately 70 sec after blue excitation light irradiation. The timing of biased cp-actin reappearance was delayed under the background of strong red light or in the absence of red light. Consistently, chloroplast movement was delayed under these conditions. In phototropin1 (phot1) mutants, acceleration of both the disappearance and reappearance of cp-actin filaments occurred, indicating an inhibitory action of phot1 on cp-actin reorganization. Avoidance movements began sooner in phot1 than in wild type plants. No reorganization of cp-actin filaments was seen in phot2 or phot1phot2 mutants lacking phot2, which is responsible for avoidance movements. Surprisingly, jac1 mutants, lacking the accumulation response, showed no avoidance movements under the whole cell irradiation condition for GFP observation. Cp-actin filaments in jac1 did not show a biased distribution, with a small or almost no transient decrease in the number. These results indicate a close association between the biased distribution of cp-actin filaments and chloroplast movement. Further, JAC1 is suggested to function in the biased cp-actin distribution by regulating their appearance and disappearance. |
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Authors:
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Satoshi Ichikawa; Noboru Yamada; Noriyuki Suetsugu; Masamitsu Wada; Akeo Kadota |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-7 |
Journal Detail:
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Title: Plant & cell physiology Volume: - ISSN: 1471-9053 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-8 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9430925 Medline TA: Plant Cell Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Biological Sciences, Graduate School of Science and Engineering, Tokyo Metropolitan University, Tokyo 192-0397, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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