| Recurring BALB/c mouse lung inflammatory responses to episodic allergen exposure. | |
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MedLine Citation:
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PMID: 23356647 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study detailed the sequence of recurring inflammatory events associated with episodic allergen exposures of mice resulting in airway hyperreactivity, sustained inflammation, goblet-cell hyperplasia, and fibrogenesis that characterize a lung with chronic asthma. Ovalbumin (OVA)-sensitized female BALB/c mice were exposed to saline-control or OVA aerosols for 1 h per day for episodes of 3 d/wk for up to 8 wk. Lung inflammation was assessed by inflammatory cell recoveries using bronchoalveolar lavages (BAL) and tissue collagenase dispersions. Cell accumulations were observed within airway submucosal and associated perivascular spaces using immunohistochemical and tinctorial staining methods. Airway responsiveness to methacholine aerosols were elevated after 2 wk and further enhanced to a sustained level after wk 4 and 8. Although by wk 8 diminished OVA-induced accumulations of eosinophils, neutrophils, and monocyte-macrophages were observed, suggesting diminished responsiveness, the BAL recovery of lymphocytes remained elevated. Airway but not perivascular lesions persisted with a proliferating cell population, epithelial goblet-cell hyperplasia, and evidence of enhanced collagen deposition. Examination of lung inflammatory cell content before the onset of the first, second, and fourth OVA exposure episodes demonstrated enhancements in residual BAL lymphocyte and BAL and tissue eosinophil recoveries with each exposure episode. Although tissue monocyte-macrophage numbers returned to baseline prior to each exposure episode, the greatest level of accumulation was observed after wk 4. These results provide the basis for establishing the inflammatory and exposure criteria by which episodic environmental exposures to allergen might result in the development of a remodeled lung in asthma. |
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Authors:
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S J Wilson; M J Harmer; R L Lee; H M Rigden; N M Doyon-Reale; K M Forman; X Gao; M W Lieh-Lai; D J P Bassett |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of toxicology and environmental health. Part A Volume: 76 ISSN: 1528-7394 ISO Abbreviation: J. Toxicol. Environ. Health Part A Publication Date: 2013 |
Date Detail:
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Created Date: 2013-01-29 Completed Date: 2013-03-26 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 100960995 Medline TA: J Toxicol Environ Health A Country: England |
Other Details:
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Languages: eng Pagination: 176-91 Citation Subset: IM |
Affiliation:
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Histochemistry Research Unit, Faculty of Medicine, University of Southampton, Southampton, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aerosols Allergens / immunology, toxicity* Animals Asthma / chemically induced*, immunology, pathology Bronchoalveolar Lavage Fluid / chemistry, cytology Cell Count Chronic Disease Collagen / metabolism Female Fibrosis / chemically induced, metabolism, pathology Inhalation Exposure / adverse effects* Leukocytes / drug effects, pathology Lung / drug effects, metabolism, pathology Methacholine Chloride / diagnostic use Mice Mice, Inbred BALB C Monocyte-Macrophage Precursor Cells / drug effects, pathology Ovalbumin / immunology, toxicity* Recurrence Respiratory Function Tests Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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5U01HD37261-04/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Aerosols; 0/Allergens; 62-51-1/Methacholine Chloride; 9006-59-1/Ovalbumin; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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