| Recruitment of host's progenitor cells to sites of human amniotic fluid stem cells implantation. | |
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MedLine Citation:
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PMID: 21459439 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The amniotic fluid is a new source of multipotent stem cells with a therapeutic potential for human diseases. Cultured at low cell density, human amniotic fluid stem cells (hAFSCs) were still able to generate colony-forming unit-fibroblast (CFU-F) after 60 doublings, thus confirming their staminal nature. Moreover, after extensive in vitro cell expansion hAFSCs maintained a stable karyotype. The expression of genes, such as SSEA-4, SOX2 and OCT3/4 was confirmed at early and later culture stage. Also, hAFSCs showed bright expression of mesenchymal lineage markers and immunoregulatory properties. hAFSCs, seeded onto hydroxyapatite scaffolds and subcutaneously implanted in nude mice, played a pivotal role in mounting a response resulting in the recruitment of host's progenitor cells forming tissues of mesodermal origin such as fat, muscle, fibrous tissue and immature bone. Implanted hAFSCs migrated from the scaffold to the skin overlying implant site but not to other organs. Given their in vivo: (i) recruitment of host progenitor cells, (ii) homing towards injured sites and (iii) multipotentiality in tissue repair, hAFSCs are a very appealing reserve of stem cells potentially useful for clinical application in regenerative medicine. |
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Authors:
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Teodelinda Mirabella; Alessandro Poggi; Monica Scaranari; Massimo Mogni; Mario Lituania; Chiara Baldo; Ranieri Cancedda; Chiara Gentili |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-04-02 |
Journal Detail:
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Title: Biomaterials Volume: 32 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-04-25 Completed Date: 2011-08-15 Revised Date: 2012-03-15 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 4218-27 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Oncology, Biology, and Genetics, University of Genova, Largo Rosanna Benzi 10, 16132 Genova, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amniotic Fluid
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cytology* Animals Biological Markers / metabolism Cell Differentiation / physiology Cell Proliferation Cells, Cultured Coculture Techniques Humans Karyotyping Leukocytes, Mononuclear / cytology, physiology Mice Mice, Nude Multipotent Stem Cells / cytology, physiology* Regenerative Medicine Stem Cells / cytology, physiology* T-Lymphocytes / physiology |
| Grant Support | |
ID/Acronym/Agency:
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GTB07001//Telethon |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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