| Recruitment of epidermal growth factor and transferrin receptors into coated pits in vitro: differing biochemical requirements. | |
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MedLine Citation:
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PMID: 8400457 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The biochemical requirements for epidermal growth factor (EGF) and transferrin receptor-mediated endocytosis were compared using perforated human A431 cells. Morphological studies showed that horseradish peroxidase (HRP)-conjugated EGF and gold-labeled antitransferrin (Tfn) receptor antibodies were colocalized during endocytosis in vitro. The sequestration of both ligands into deeply invaginated coated pits required ATP hydrolysis and cytosolic factors and was inhibited by GTP gamma S, indicating mechanistic similarities. Importantly, several differences in the biochemical requirements for sequestration of EGF and Tfn were also detected. These included differing requirements for soluble AP (clathrin assembly protein) complexes, differing cytosolic requirements, and differing sensitivities to the tyrosine kinase inhibitor, genistein. The biochemical differences detected between EGF and Tfn sequestration most likely reflect specific requirements for the recruitment of EGF-receptors (R) into coated pits. This assay provides a novel means to identify the molecular bases for these biochemical distinctions and to elucidate the mechanisms involved in ligand-induced recruitment of EGF-R into coated pits. |
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Authors:
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C Lamaze; T Baba; T E Redelmeier; S L Schmid |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular biology of the cell Volume: 4 ISSN: 1059-1524 ISO Abbreviation: Mol. Biol. Cell Publication Date: 1993 Jul |
Date Detail:
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Created Date: 1993-11-16 Completed Date: 1993-11-16 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 715-27 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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analogs & derivatives,
metabolism,
pharmacology Adenylyl Imidodiphosphate / pharmacology Carcinoma, Squamous Cell Cell Membrane Permeability Coated Pits, Cell-Membrane / drug effects, metabolism*, ultrastructure Cytosol / metabolism Egtazic Acid / pharmacology Endocytosis* / drug effects Enzyme-Linked Immunosorbent Assay Epidermal Growth Factor / metabolism* Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology Humans Kinetics Ligands Microscopy, Immunoelectron Receptor, Epidermal Growth Factor / analysis, metabolism* Receptors, Transferrin / analysis, metabolism* Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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CA27489/CA/NCI NIH HHS; GM42445/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ligands; 0/Receptors, Transferrin; 25612-73-1/Adenylyl Imidodiphosphate; 35094-46-3/adenosine 5'-O-(3-thiotriphosphate); 37589-80-3/Guanosine 5'-O-(3-Thiotriphosphate); 56-65-5/Adenosine Triphosphate; 62229-50-9/Epidermal Growth Factor; 67-42-5/Egtazic Acid; EC 2.7.10.1/Receptor, Epidermal Growth Factor |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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