| Recruitment of cellular prion protein to mitochondrial raft-like microdomains contributes to apoptosis execution. | |
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MedLine Citation:
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PMID: 22031292 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We examined the possibility that cellular prion protein (PrP(C)) plays a role in the receptor-mediated apoptotic pathway. We first found that CD95/Fas triggering induced a redistribution of PrP(C) to the mitochondria of T lymphoblastoid CEM cells via a mechanism that brings into play microtubular network integrity and function. In particular, we demonstrated that PrP(C) was redistributed to raft-like microdomains at the mitochondrial membrane, as well as at endoplasmic reticulum-mitochondria-associated membranes. Our in vitro experiments also demonstrated that, although PrP(C) had such an effect on mitochondria, it induced the loss of mitochondrial membrane potential and cytochrome c release only after a contained rise of calcium concentration. Finally, the involvement of PrP(C) in apoptosis execution was also analyzed in PrP(C)-small interfering RNA-transfected cells, which were found to be significantly less susceptible to CD95/Fas-induced apoptosis. Taken together, these results suggest that PrP(C) might play a role in the complex multimolecular signaling associated with CD95/Fas receptor-mediated apoptosis. |
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Authors:
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Vincenzo Mattei; Paola Matarrese; Tina Garofalo; Antonella Tinari; Lucrezia Gambardella; Laura Ciarlo; Valeria Manganelli; Vincenzo Tasciotti; Roberta Misasi; Walter Malorni; Maurizio Sorice |
Publication Detail:
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Type: Journal Article Date: 2011-10-26 |
Journal Detail:
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Title: Molecular biology of the cell Volume: 22 ISSN: 1939-4586 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-15 Completed Date: 2012-07-30 Revised Date: 2013-05-23 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 4842-53 Citation Subset: IM |
Affiliation:
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Laboratory of Experimental Medicine and Environmental Pathology, Sabina Universitas, 02100 Rieti, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD95
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genetics,
metabolism* Apoptosis / physiology* Calcium / metabolism Cell Line, Tumor Cytochromes c / genetics, metabolism Humans Membrane Microdomains / genetics, metabolism* Microtubules / genetics, metabolism Mitochondrial Membranes / metabolism* PrPC Proteins / genetics, metabolism* RNA, Small Interfering / genetics Signal Transduction / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD95; 0/FAS protein, human; 0/PrPC Proteins; 0/RNA, Small Interfering; 7440-70-2/Calcium; 9007-43-6/Cytochromes c |
| Comments/Corrections | |
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