| Recruitment of apoptotic cysteine proteases (caspases) in influenza virus-induced cell death. | |
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MedLine Citation:
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PMID: 10338194 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Influenza virus infection induces apoptosis in cultured cells with an augmented expression of Fas (APO-1/CD95). Caspases, a family of cysteine proteases structurally related to interleukin-1-beta-converting enzyme (ICE), play crucial roles in apoptosis induced by various stimuli, including Fas. However, activation of the caspase-cascade seems to be different in various pathways of apoptotic stimuli. We therefore examined the involvement of caspases in influenza virus-induced apoptosis using caspase inhibitors. We found that z-VAD-fmk and z-IETD-fmk effectively inhibited virus-induced apoptosis, whereas Ac-DEVD-CHO and Ac-YVAD-CHO showed partial and little effect on virus-induced cell death, respectively. Consistently, caspase-3-like activity, but not caspase-1-like activity, was increased in the virus-infected cells. The transfection of plasmids encoding viral inhibitors of caspase (v-FLIP or crmA) into HeLa cells inhibited apoptosis by virus infection. The peptide inhibitors of caspases used in this study did not inhibit viral replication. We conclude that influenza virus infection activates some caspases, and that this activation may be downstream of viral replication. |
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Authors:
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T Takizawa; C Tatematsu; K Ohashi; Y Nakanishi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Microbiology and immunology Volume: 43 ISSN: 0385-5600 ISO Abbreviation: Microbiol. Immunol. Publication Date: 1999 |
Date Detail:
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Created Date: 1999-09-03 Completed Date: 1999-09-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7703966 Medline TA: Microbiol Immunol Country: JAPAN |
Other Details:
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Languages: eng Pagination: 245-52 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Institute for Developmental Research, Aichi Human Service Center, Kasugai, Japan. takizawa@inst-hsc.pref.aichi.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis* Caspases / antagonists & inhibitors, metabolism* Enzyme Activation Enzyme Inhibitors Hela Cells Humans Influenza A virus / physiology* Virus Replication |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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