Document Detail


Recovery, viability and clinical toxicity of thawed and washed haematopoietic progenitor cells: analysis of 952 autologous peripheral blood stem cell transplantations.
MedLine Citation:
PMID:  17724443     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cryopreservation and thawing of haematopoietic stem cells are associated with cell loss and infusion-related toxicities. We analysed viability, total nucleated cell (TNC) and CD34+ cell recovery, and infusion-related toxicities of 952 thawed and washed products. Mean TNC and CD34+ viable cells recoveries were 55.9+/-18.6 and 98.0+/-36.5%, respectively. Mean cell viability was 68.25+/-18.9%. TNC recovery was correlated with viability but independent of the initial nucleated cell concentration. No difference in TNC recovery or viability was observed according to underlying diseases, except for myeloma, for which these variables were significantly lower (P<0.05). CD34+ cell recovery was not correlated with viability or CD34+ initial count and was similar for all diseases. Cryostorage duration was not associated with cell loss. Immediate adverse events occurred in 169 patients (19%) and were moderate (grade I or II) for the majority of patients. Clinical toxicity was associated with a higher infused cell number and the presence of clumps in infused bags. The washing procedure of cell products lead to a low rate of adverse events, but patients transplanted with high cell numbers or bags in which clumps were identified are predisposed to such complications.
Authors:
E Foïs; M Desmartin; S Benhamida; F Xavier; V Vanneaux; D Rea; J-P Fermand; B Arnulf; N Mounier; M Ertault; J-P Lotz; L Galicier; E Raffoux; M Benbunan; J-P Marolleau; J Larghero
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Publication Detail:
Type:  Journal Article     Date:  2007-08-27
Journal Detail:
Title:  Bone marrow transplantation     Volume:  40     ISSN:  0268-3369     ISO Abbreviation:  Bone Marrow Transplant.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-17     Completed Date:  2007-12-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8702459     Medline TA:  Bone Marrow Transplant     Country:  England    
Other Details:
Languages:  eng     Pagination:  831-5     Citation Subset:  IM    
Affiliation:
Unité de Thérapie Cellulaire, Departement de Biotherapic, Assistance Publique-Hôpitaux de Paris, Hôpital Saint Louis, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD34
Cell Count
Cell Survival
Cryopreservation / methods*
Hematologic Diseases / complications,  therapy
Hematopoietic Stem Cells / cytology*
Humans
Neoplasms / complications,  therapy
Peripheral Blood Stem Cell Transplantation / adverse effects*,  methods
Retrospective Studies
Risk Factors
Transplantation, Autologous
Chemical
Reg. No./Substance:
0/Antigens, CD34

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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