Document Detail


Recovery and viability of Dirofilaria immitis microfilariae.
MedLine Citation:
PMID:  6209969     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The viability of Dirofilaria immitis microfilariae recovered from canine blood by different methods was determined. Microfilaria recovery techniques included saponin lysis, saponin lysis with a trypsin treatment, dextran sedimentation and phytohemagglutinin treatment. Criteria for evaluating viability were microfilarial motility in vitro at 37 degrees C, microfilarial development in mosquitoes and the ability of microfilariae to circulate in mice. Although each method produced motile microfilariae, differences among groups of microfilariae recovered by different techniques were apparent by each of the criteria for viability. Saponin lysis gave superior yields of viable microfilariae.
Authors:
R B Grieve; M Mika-Grieve; J B Lok; T F Marchell; E W Cupp
Related Documents :
21417799 - Impact of dietary gold nanoparticles in zebrafish at very low contamination pressure: t...
24702869 - Detection of 20 kda and 32 kda ige-binding proteins as the major allergens in italian s...
1141169 - Collaborative study of a method for the isolation of light filth from breading of froze...
21185549 - Antihypertensive peptides: production, bioavailability and incorporation into foods.
18576989 - Attenuated total reflectance-fourier transform infrared spectroscopy coupled with multi...
3391969 - Determination of sulfur amino acids and tryptophan in foods and food and feed ingredien...
4095189 - Effects of pre-feeding on food-approach latency and food consumption speed in food depr...
17269139 - Discrete crosslinked fibrin microthread scaffolds for tissue regeneration.
23911759 - Subtyping of listeria monocytogenes isolates recovered from retail ready-to-eat foods, ...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Acta tropica     Volume:  41     ISSN:  0001-706X     ISO Abbreviation:  Acta Trop.     Publication Date:  1984 Sep 
Date Detail:
Created Date:  1985-01-09     Completed Date:  1985-01-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370374     Medline TA:  Acta Trop     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  271-8     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aedes / parasitology
Animals
Blood / parasitology
Culture Media
Dextrans / pharmacology
Dirofilaria immitis* / pathogenicity,  physiology
Dirofilariasis / veterinary*
Dog Diseases / parasitology*
Dogs
Female
Filarioidea* / physiology
Hemolysis
Mice
Mice, Inbred Strains
Microfilaria
Phytohemagglutinins / pharmacology
Saponins / pharmacology
Trypsin / pharmacology
Grant Support
ID/Acronym/Agency:
223-82-7002//PHS HHS; AI-18249/AI/NIAID NIH HHS; AI-92619/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Phytohemagglutinins; 0/Saponins; 0/phytohemagglutinin-P; 9004-54-0/Dextrans; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The human thymic microenvironment.
Next Document:  A case of renal carcinoma with bone metastasis, producing alpha-fetoprotein