Document Detail

Recovery and viability of Dirofilaria immitis microfilariae.
MedLine Citation:
PMID:  6209969     Owner:  NLM     Status:  MEDLINE    
The viability of Dirofilaria immitis microfilariae recovered from canine blood by different methods was determined. Microfilaria recovery techniques included saponin lysis, saponin lysis with a trypsin treatment, dextran sedimentation and phytohemagglutinin treatment. Criteria for evaluating viability were microfilarial motility in vitro at 37 degrees C, microfilarial development in mosquitoes and the ability of microfilariae to circulate in mice. Although each method produced motile microfilariae, differences among groups of microfilariae recovered by different techniques were apparent by each of the criteria for viability. Saponin lysis gave superior yields of viable microfilariae.
R B Grieve; M Mika-Grieve; J B Lok; T F Marchell; E W Cupp
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Acta tropica     Volume:  41     ISSN:  0001-706X     ISO Abbreviation:  Acta Trop.     Publication Date:  1984 Sep 
Date Detail:
Created Date:  1985-01-09     Completed Date:  1985-01-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370374     Medline TA:  Acta Trop     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  271-8     Citation Subset:  IM    
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MeSH Terms
Aedes / parasitology
Blood / parasitology
Culture Media
Dextrans / pharmacology
Dirofilaria immitis* / pathogenicity,  physiology
Dirofilariasis / veterinary*
Dog Diseases / parasitology*
Filarioidea* / physiology
Mice, Inbred Strains
Phytohemagglutinins / pharmacology
Saponins / pharmacology
Trypsin / pharmacology
Grant Support
223-82-7002//PHS HHS; AI-18249/AI/NIAID NIH HHS; AI-92619/AI/NIAID NIH HHS
Reg. No./Substance:
0/Culture Media; 0/Phytohemagglutinins; 0/Saponins; 0/phytohemagglutinin-P; 9004-54-0/Dextrans; EC

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