| Recovery and properties of a fructooligosaccharides-producing beta-fructofuranosidase from Aspergillus japonicus CCRC 38011. | |
| | |
MedLine Citation:
|
PMID: 7809276 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The fungus (Aspergillus japonicus CCRC 38011) was found to be able to produce beta-fructofuranosidase with high transfructosylating activity (Ut), a key enzyme involved in synthesis of fructooligosaccharides from sucrose. The Ut productivities of this microorganism were 191.5 units/ml broth in a 6-L jar-fermentor and 256.1 units/ml broth in a 1000-L pilot-fermentor in a modified medium containing 8% sucrose as a carbon source. Most of the Ut of this microorganism was found to be bound with mycelia. Incubation and sonication treatment extracted this enzyme from mycelia with maximum efficiencies of 66.3% and 44.3%, respectively. On the other hand, homogenization and freeze-thawing treatment had only a small effect on enzyme extraction. The soluble enzyme extracted from mycelia by incubation at pH 5.0 and 40 degrees C for 3 hours could be easily recovered and purified by acetone precipitation. The recovery of Ut from the crude enzyme solution was 99.5% by mixing the solution with an equal volume of acetone at 4 degrees C, followed by centrifugation. The purification factor of acetone precipitation was 15.8. The optimum pH and temperature of Ut was 5.0 and 65-70 degrees C, respectively. The enzyme was stable at a pH between 4.0 and 5.0 and at a temperature below 60 degrees C. |
| | |
Authors:
|
Y C Su; C S Sheu |
Related Documents
:
|
8092996 - Purification and characterization of an alpha-l-arabinofuranosidase from streptomyces l... 20681636 - Purification of peroxidase from horseradish (armoracia rusticana) roots. 18600646 - Hydrolysis of inulin: a kinetic study of the reaction catalyzed by an inulinase from as... 20047516 - Evaluation of various ions and compounds on nitrilase produced from streptomyces sp. 3302196 - Cryptococcal udp-glucose dehydrogenase: enzymic control of capsular biosynthesis. 23494216 - Purification of acetylcholinesterase by 9-amino-1,2,3,4-tetrahydroacridine from human e... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Proceedings of the National Science Council, Republic of China. Part B, Life sciences Volume: 17 ISSN: 0255-6596 ISO Abbreviation: Proc. Natl. Sci. Counc. Repub. China B Publication Date: 1993 Apr |
Date Detail:
|
Created Date: 1995-01-31 Completed Date: 1995-01-31 Revised Date: 2008-02-26 |
Medline Journal Info:
|
Nlm Unique ID: 8502426 Medline TA: Proc Natl Sci Counc Repub China B Country: TAIWAN |
Other Details:
|
Languages: eng Pagination: 62-9 Citation Subset: IM |
Affiliation:
|
Department of Agricultural Chemistry, National Taiwan University, Taipei, Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aspergillus
/
enzymology* Chromatography, High Pressure Liquid Fructose Glycoside Hydrolases / isolation & purification, metabolism* Kinetics Oligosaccharides / biosynthesis*, isolation & purification Thermodynamics beta-Fructofuranosidase |
| Chemical | |
Reg. No./Substance:
|
0/Oligosaccharides; 30237-26-4/Fructose; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.26/beta-Fructofuranosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Protamine-induced acute lung injury and the protective effect of agents that increase cAMP.
Next Document: Antioxidant properties of fractions and polyphenol constituents from green, oolong and black teas.