Document Detail


Recovery and properties of a fructooligosaccharides-producing beta-fructofuranosidase from Aspergillus japonicus CCRC 38011.
MedLine Citation:
PMID:  7809276     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The fungus (Aspergillus japonicus CCRC 38011) was found to be able to produce beta-fructofuranosidase with high transfructosylating activity (Ut), a key enzyme involved in synthesis of fructooligosaccharides from sucrose. The Ut productivities of this microorganism were 191.5 units/ml broth in a 6-L jar-fermentor and 256.1 units/ml broth in a 1000-L pilot-fermentor in a modified medium containing 8% sucrose as a carbon source. Most of the Ut of this microorganism was found to be bound with mycelia. Incubation and sonication treatment extracted this enzyme from mycelia with maximum efficiencies of 66.3% and 44.3%, respectively. On the other hand, homogenization and freeze-thawing treatment had only a small effect on enzyme extraction. The soluble enzyme extracted from mycelia by incubation at pH 5.0 and 40 degrees C for 3 hours could be easily recovered and purified by acetone precipitation. The recovery of Ut from the crude enzyme solution was 99.5% by mixing the solution with an equal volume of acetone at 4 degrees C, followed by centrifugation. The purification factor of acetone precipitation was 15.8. The optimum pH and temperature of Ut was 5.0 and 65-70 degrees C, respectively. The enzyme was stable at a pH between 4.0 and 5.0 and at a temperature below 60 degrees C.
Authors:
Y C Su; C S Sheu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings of the National Science Council, Republic of China. Part B, Life sciences     Volume:  17     ISSN:  0255-6596     ISO Abbreviation:  Proc. Natl. Sci. Counc. Repub. China B     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1995-01-31     Completed Date:  1995-01-31     Revised Date:  2008-02-26    
Medline Journal Info:
Nlm Unique ID:  8502426     Medline TA:  Proc Natl Sci Counc Repub China B     Country:  TAIWAN    
Other Details:
Languages:  eng     Pagination:  62-9     Citation Subset:  IM    
Affiliation:
Department of Agricultural Chemistry, National Taiwan University, Taipei, Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Aspergillus / enzymology*
Chromatography, High Pressure Liquid
Fructose
Glycoside Hydrolases / isolation & purification,  metabolism*
Kinetics
Oligosaccharides / biosynthesis*,  isolation & purification
Thermodynamics
beta-Fructofuranosidase
Chemical
Reg. No./Substance:
0/Oligosaccharides; 30237-26-4/Fructose; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.26/beta-Fructofuranosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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