| Recovery from vitamin B-12-induced unbalanced growth. The shortened cell cycle and the deoxyribonucleoside triphosphate pools. | |
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MedLine Citation:
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PMID: 417723 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The deoxyribonucleoside triphosphate pools are undetectable in vitamin B-12-deficient cells of Euglena gracillis, but appear rapidly after the replenishment with the vitamin. They reach a maximum size that is about 6 times that of normal exponentially growing cells, but decrease to almost zero as the cells divide. The pools expand again during the post-replenishment shortened cell cycle. However, the expansion takes place during rather than before the resumption of DNA synthesis. The maximum sizes reached are still larger than in normal cells. By using the protein-synthesis inhibitor cycloheximide and determining the pool size, we found that vitamin-deficient cells apparently accumulate a large amount of ribonucleoside triphosphate reductase apoenzyme, which lacks the vitamin B12 coenzyme. We showed that the production of the deoxyribonucleoside triphosphates is not closely coupled to DNA synthesis under our experimental conditions, and that the concentration of the deoxyribonucleoside triphosphate pools per unit of DNA synthesized is almost constant for all stages of growth examined. |
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Authors:
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G H Goetz; E F Carell |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Biochemical journal Volume: 170 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1978 Mar |
Date Detail:
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Created Date: 1978-06-12 Completed Date: 1978-06-12 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 631-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Count Cell Cycle Cycloheximide / pharmacology DNA / biosynthesis Deoxyribonucleotides / biosynthesis, metabolism* Euglena gracilis / drug effects, growth & development, metabolism* Hydroxyurea / pharmacology Kinetics Vitamin B 12 / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Deoxyribonucleotides; 127-07-1/Hydroxyurea; 66-81-9/Cycloheximide; 68-19-9/Vitamin B 12; 9007-49-2/DNA |
| Comments/Corrections | |
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