Document Detail


Recovery of clostridia on catalase-treated plating media.
MedLine Citation:
PMID:  869526     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Four plating media commonly used for culturing clostridia were tested for their ability to support growth of several Clostridium species after storage of the plates for 1 to 10 days at 4 and 25 degrees C with and without subsequent addition of catalase. Liver-veal (LV) agar and brain heart infusion (BHI) agar rapidly became incapable of supporting growth after storage without added catalase, whereas Shahidi Ferguson perfringens agar base and Brewer anaerobic agar were less affected. Plate counts of vegetative cells of nine of the less fastidious Clostridium species on untreated LV and BHI agars, stored for 3 days at 4 degrees C, were 60 to 90% lower than counts on catalase-treated media. Counts on Shahidi Ferguson perfringens agar base were only 1 to 24% lower on untreated medium with the same species. Addition of 500 U of purified beef liver catalase to the surface of the 3-day-old agars before inoculation resulted in substantial restoration of the ability of the media to support colony formation from vegetative cells except with the most strictly anaerobic species (nonproteolytic C. botulinum types B, E, and F, and C. novyii types A and B). A similar response was obtained with spores of the less fastidious species on catalase-treated media. Our results suggest that inhibition of most Clostridium species on LV and BHI agars may be due to accumulation of peroxide during preparation, storage, and incubation of the media, and also suggest that the presence of glucose in these media is a major factor contributing to their inability to support growth. It is believed that the addition of exogenous catalase prevents the accumulation of peroxide(s), thus allowing colony formation from vegetative cells of the clostridia under what would otherwise be unsuitable cultural conditions.
Authors:
S M Harmon; D A Kautter
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  33     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1977 Apr 
Date Detail:
Created Date:  1977-07-18     Completed Date:  1977-07-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  762-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Catalase*
Clostridium / drug effects,  growth & development,  isolation & purification*
Culture Media*
Evaluation Studies as Topic
Hot Temperature
Hydrogen Peroxide / pharmacology
Species Specificity
Spores, Bacterial / isolation & purification
Chemical
Reg. No./Substance:
0/Culture Media; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.6/Catalase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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