Document Detail

Recovery of cell volume and electrolytes of A6 cells after re-establishing isotonicity following hypotonic stress.
MedLine Citation:
PMID:  12898258     Owner:  NLM     Status:  MEDLINE    
Cellular element concentrations and dry weight contents in A6 cells were determined using electron microprobe analysis to establish whether these cells exhibit a regulatory volume increase (post-RVD-RVI) when re-establishing isotonicity following a hypotonically induced regulatory volume decrease (RVD). Hypotonic stress was induced by reducing basolateral [NaCl], and hence, osmolarity fell from 260 to 140 mosmol/l. The alterations in cell volume after re-establishing isotonicity, calculated from the cellular dry weight changes, indicate within the first 2 min cell shrinkage from 120 to 76% of control, compatible with almost ideal osmometric behaviour of A6 cells, and thereafter a post-RVD-RVI to 94%. The cellular uptake of osmolytes necessary to explain the post-RVD-RVI could be accounted for solely by a gain in cellular K and Cl. The involvement of a Na-K-2Cl cotransporter in most of the KCl uptake seems plausible since basolateral bumetanide blocked KCl uptake and post-RVD-RVI. The net uptake of cations (K uptake of 185.2, Na loss of 8.2 mmol/kg dry wt) during the isotonic period exceeded the Cl uptake by 38.2 mmol/kg dry wt, suggesting the uptake of another anion and/or the alteration of cellular buffer capacity. The relatively low Na concentration maintained during the isotonic period (13.3 vs. 20.4 mmol/kg wet wt under control conditions) might favour electrolyte uptake via the Na-K-2Cl cotransporter.
T Grosse; I Heid; I Oztürk; S Borgmann; F X Beck; A Dörge
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-07-26
Journal Detail:
Title:  Pflügers Archiv : European journal of physiology     Volume:  447     ISSN:  0031-6768     ISO Abbreviation:  Pflugers Arch.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2004-02-20     Completed Date:  2004-09-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0154720     Medline TA:  Pflugers Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  29-34     Citation Subset:  IM    
Physiologisches Institut, Pettenkoferstrasse 12, 80336 Munich, Germany.
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MeSH Terms
Cell Line, Transformed
Cell Size / drug effects,  physiology
Electrolytes / analysis*,  metabolism
Hypotonic Solutions / analysis*,  metabolism
Isotonic Solutions / analysis*,  metabolism
Osmolar Concentration
Stress, Physiological / metabolism*
Xenopus laevis
Reg. No./Substance:
0/Electrolytes; 0/Hypotonic Solutions; 0/Isotonic Solutions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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