| Recovery of cell cycle delay following targeted gene repair by oligonucleotides. | |
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MedLine Citation:
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PMID: 17560837 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously shown that activation of the homologous recombinational repair pathway leads to a block of cell division in corrected cells, possibly through the activity of checkpoint proteins Chk1 and Chk2. In this study, we examine the long-term impact of this stalling on the growth of cells that have enabled gene repair events. Using a mutated eGFP gene as an episomal reporter, we show that corrected (eGFP-positive) cells contain only a few active replication templates 2 weeks after electroporation, yet do not display an apoptotic or senescent phenotype. By 6 weeks after electroporation, cells resume active replication with a cell cycle profile that is comparable to that of the non-corrected (eGFP-negative) population. These results indicate that the initial stalling is transient and eGFP-positive cells eventually resume a normal phenotypic growth pattern, allowing for passaging and expansion in vitro. |
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Authors:
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Luciana Ferrara; Julia U Engstrom; Timothy Schwartz; Hetal Parekh-Olmedo; Eric B Kmiec |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-06-11 |
Journal Detail:
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Title: DNA repair Volume: 6 ISSN: 1568-7864 ISO Abbreviation: DNA Repair (Amst.) Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-09-17 Completed Date: 2007-12-19 Revised Date: 2011-02-25 |
Medline Journal Info:
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Nlm Unique ID: 101139138 Medline TA: DNA Repair (Amst) Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1529-35 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, University of Delaware, Delaware Biotechnology Institute, 15 Innovation Way, Newark, DE 19711, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cell Cycle / drug effects* DNA Repair* DNA Replication Oligonucleotides / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA 89325-01A1/CA/NCI NIH HHS; R01 CA089325-05A2/CA/NCI NIH HHS; R01 CA089325-06/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Oligonucleotides |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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