Document Detail

Recovery and amplification of placental RNA from dried maternal blood spots: utility for non-invasive prenatal diagnosis.
MedLine Citation:
PMID:  17007679     Owner:  NLM     Status:  MEDLINE    
Methods utilizing circulating cell-free RNA in plasma have clinical applications for cancer and prenatal genetic analysis. Given these potential roles, the feasibility of detecting placental specific RNA in dried maternal blood spots after storage at room temperature for varying lengths of time was investigated. Using real-time polymerase chain reaction (PCR), positive amplification of placental-specific beta-human chorionic gonadotrophin transcripts was demonstrated in nine of 11 dried blood samples from first and second trimester pregnancies stored at room temperature for up to 4 weeks. This work demonstrates feasibility in isolation and amplification of placental mRNA using dried maternal blood spots. With the development of fetal and placental RNA markers, this approach would allow simplified collection, transport, and storage of samples for prenatal genetic diagnosis and pregnancy related complications.
Carolina J Jorgez; Joe Leigh Simpson; Farideh Z Bischoff
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  13     ISSN:  1472-6483     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-29     Completed Date:  2006-11-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  558-61     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, TX, USA.
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MeSH Terms
Chorionic Gonadotropin, beta Subunit, Human / blood,  genetics
Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / genetics
Nucleic Acid Amplification Techniques / methods*
Placenta / physiology*
Pregnancy Trimester, First
Pregnancy Trimester, Second
Prenatal Diagnosis / methods*
RNA / analysis,  blood*,  isolation & purification*
Grant Support
Reg. No./Substance:
0/Chorionic Gonadotropin, beta Subunit, Human; 63231-63-0/RNA; EC 3-Phosphate Dehydrogenase (NADP+)

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