Document Detail

Recovery of Bordetella pertussis from PCR-positive nasopharyngeal samples is dependent on bacterial load.
MedLine Citation:
PMID:  23035189     Owner:  NLM     Status:  MEDLINE    
Viable Bordetella pertussis isolates are essential for surveillance purposes. We performed culture of 223 PCR-positive nasopharyngeal samples. B. pertussis was recovered from 45 (20.2%) of the samples. Growth was associated with a high bacterial load, as determined by PCR. Culture from PCR-positive samples is a feasible approach to recover B. pertussis isolates, and culture can be limited to samples with a high bacterial load.
Didrik F Vestrheim; Martin Steinbakk; Martha L Bjørnstad; Amir Moghaddam; Nils Reinton; Mette L Dahl; Nils Grude; Per Sandven
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2012-10-03
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  50     ISSN:  1098-660X     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-19     Completed Date:  2013-04-17     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4114-5     Citation Subset:  IM    
Department of Bacteriology and Immunology, Norwegian Institute of Public Health, Oslo, Norway.
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MeSH Terms
Aged, 80 and over
Bacterial Load*
Bacteriological Techniques / methods*
Bordetella pertussis / genetics,  growth & development,  isolation & purification*
Child, Preschool
Infant, Newborn
Middle Aged
Nasopharynx / microbiology*
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Whooping Cough / diagnosis*,  microbiology
Young Adult

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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