Document Detail


Recovery of Bordetella pertussis from PCR-positive nasopharyngeal samples is dependent on bacterial load.
MedLine Citation:
PMID:  23035189     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Viable Bordetella pertussis isolates are essential for surveillance purposes. We performed culture of 223 PCR-positive nasopharyngeal samples. B. pertussis was recovered from 45 (20.2%) of the samples. Growth was associated with a high bacterial load, as determined by PCR. Culture from PCR-positive samples is a feasible approach to recover B. pertussis isolates, and culture can be limited to samples with a high bacterial load.
Authors:
Didrik F Vestrheim; Martin Steinbakk; Martha L Bjørnstad; Amir Moghaddam; Nils Reinton; Mette L Dahl; Nils Grude; Per Sandven
Related Documents :
24494069 - The effect of platelet rich plasma on chondrogenic differentiation of human adipose der...
23737099 - Chondrogenic potential of human dermal fibroblasts in a contractile, soft, self-assembl...
23949019 - In vitro culture system for keratinocytes obtained from oral lichen planus lesions.
24106119 - A new human primary epithelial cell culture model to study conjunctival inflammation.
18783439 - Nuclear translocation and dnase i-like enzymatic activity of acinetobacter baumannii ou...
861949 - Stimulation of dna synthesis by human placental lactogen or insulin in organ cultures o...
5325419 - Injury and growth of serratia marcescens studied in a thermal gradient incubator.
2113829 - Neutral red (nr) assay for cell viability and xenobiotic-induced cytotoxicity in primar...
2115339 - Effect of extracellular matrix on prolactin secretion and mrna accumulation in gh3 cells.
Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2012-10-03
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  50     ISSN:  1098-660X     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-19     Completed Date:  2013-04-17     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4114-5     Citation Subset:  IM    
Affiliation:
Department of Bacteriology and Immunology, Norwegian Institute of Public Health, Oslo, Norway. didrik.frimann.vestrheim@fhi.no
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Aged, 80 and over
Bacterial Load*
Bacteriological Techniques / methods*
Bordetella pertussis / genetics,  growth & development,  isolation & purification*
Child
Child, Preschool
Female
Humans
Infant
Infant, Newborn
Male
Middle Aged
Nasopharynx / microbiology*
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Whooping Cough / diagnosis*,  microbiology
Young Adult
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Identification of Streptococcus intermedius central nervous system infection by use of PCR and elect...
Next Document:  Direct detection and genotyping of Klebsiella pneumoniae carbapenemases from urine by use of a new D...