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Recordings from cultured newt olfactory receptor cells.
MedLine Citation:
PMID:  22559969     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Freshly dissociated olfactory receptor cells (ORCs) are commonly used in electrophysiological research investigations of the physicochemical mechanisms of olfactory signal transduction. Because the morphology of cultured cells clearly becomes worse over time, the ORCs are examined traditionally within several days after dissociation. However, there has been a major concern that cells are affected soon after dissociation. To gain a better understanding of the reliability of data obtained from solitary cells, we obtained electrical data during the lifetime of single ORCs dissociated from the newt. The time course for the deterioration could be revealed by monitoring the membrane properties during culture. Although the number of living cells that were identified by trypan blue extrusion declined day by day, the remaining cells retained morphology and their fundamental electrical features until day 19. In some cells, the cilia and dendrite were observed until day 21, and the bipolar morphology until day 31. The fundamental features of cell excitation were maintained during culture without showing remarkable changes when they retained morphological features. The results suggest that electrical properties of cells are almost unchanged within several days. Furthermore, the dissociated newt ORCs can be used for several weeks that are almost comparable to the intrinsic lifetime of the ORCs in vivo.
Authors:
Kyohei Matsumura; Masahiro Matsumoto; Takashi Kurahashi; Hiroko Takeuchi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Zoological science     Volume:  29     ISSN:  0289-0003     ISO Abbreviation:  Zool. Sci.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8702287     Medline TA:  Zoolog Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  340-5     Citation Subset:  IM    
Affiliation:
1 Graduate School of Frontier Biosciences, Osaka University, Toyonaka, Osaka 560-8531, Japan.
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