| Reconstruction of degenerate rd mouse retina by transplantation of transgenic photoreceptors. | |
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MedLine Citation:
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PMID: 1639605 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Photoreceptors from neonatal transgenic mice with normally developing retinas were transplanted to the subretinal spaces of 2-3-month-old rd mutant mice that lack photoreceptors. The transgenic mouse photoreceptors express high levels of the lac Z reporter gene product, beta-galactosidase, which facilitated tracking the transplanted cells. Two sources were used for these cells: (1) dissection of retinal microaggregates containing photoreceptors and (2) papain-dissociated photoreceptors. Host retinas were examined after transplantation. Both methods led to survival of photoreceptors for at least 2 mo after transplantation. Relatively mature outer segments were found only in transplanted microaggregates; this occurred optimally when the cells were adjacent to the retinal pigment epithelium (RPE). beta-galactosidase-labeled outer segments associated closely with the apical processes of the host RPE, which, together with labeled phagosomes in the RPE cells, suggested functional interaction between the transplanted photoreceptors and the host RPE. This study is the first to the authors' knowledge to show electron microscopically that a morphologically normal-appearing photoreceptor layer can be reconstructed in an otherwise photoreceptorless retina. |
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Authors:
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P Gouras; J Du; H Kjeldbye; S Yamamoto; D J Zack |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 33 ISSN: 0146-0404 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 1992 Aug |
Date Detail:
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Created Date: 1992-08-31 Completed Date: 1992-08-31 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2579-86 Citation Subset: IM |
Affiliation:
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Edward S. Harkness Eye Institute, Columbia University, New York, New York 10032. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Female Male Mice Mice, Mutant Strains Mice, Transgenic Photoreceptor Cells / enzymology, transplantation* Pigment Epithelium of Eye / enzymology, ultrastructure Retina / ultrastructure Retinal Degeneration / pathology* Rod Cell Outer Segment / enzymology, ultrastructure beta-Galactosidase / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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