| Reconstitution of stratified murine and human oesophageal epithelia in an in vivo transplant culture system. | |
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MedLine Citation:
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PMID: 18609138 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The molecular and cellular events responsible for regulating development of the oesophageal epithelium are not well understood. At least in part, this is due to the lack of a suitable model system with which to study the process. Here, we report development of a manipulable in vivo transplant model for mouse or human oesophageal epithelium. MATERIAL AND METHODS: Epithelial cells were isolated from mouse or human oesophagus and inoculated into de-epithelialized and devitalized rat tracheas. The rat trachea, containing cells, was placed subcutaneously under the dorsal skin of immunodeficient mice. RESULTS: We show that a multilayered stratified squamous epithelium can be generated in 4-6 weeks from as few as 5 x 10(4) isolated oesophageal epithelial cells. The reconstituted epithelium recapitulates many of the structural and histological features of the normal oesophageal epithelium, including a basal layer of cuboidal-like cells, suprabasal layers of differentiating squamous cells and, in the case of murine cells, a superficial layer of cornified material. CONCLUSION: Our model can be used to generate a multilayered normal murine or human epithelium from a single cell suspension of oesophageal epithelial cells. The ability to genetically manipulate the cells prior to growth in the model is a powerful tool with which to study the molecular mechanisms involved in the development of normal oesophagus or in pathogenic processes such as Barrett's metaplasia or tumorigenesis. |
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Authors:
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Daniel Croagh; Shuli Cheng; Anjali Tikoo; Sanjay Nandurkar; Robert J S Thomas; Pritinder Kaur; Wayne A Phillips |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Scandinavian journal of gastroenterology Volume: 43 ISSN: 1502-7708 ISO Abbreviation: Scand. J. Gastroenterol. Publication Date: 2008 |
Date Detail:
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Created Date: 2008-09-19 Completed Date: 2009-02-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0060105 Medline TA: Scand J Gastroenterol Country: Norway |
Other Details:
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Languages: eng Pagination: 1158-68 Citation Subset: IM |
Affiliation:
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Peter MacCallum Cancer Centre, Surgical Oncology Research Laboratory, Melbourne, Victoria, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Culture Techniques Cell Differentiation Cells, Cultured Epithelial Cells / cytology* Epithelium / transplantation Esophagus / cytology* Humans Mice Mice, Inbred C57BL Models, Biological Rats Rats, Sprague-Dawley |
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