| Reconstitution of novel mitochondrial uncoupling proteins UCP2 and UCP3. | |
| | |
MedLine Citation:
|
PMID: 12418549 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Reconstitution of novel mitochondrial uncoupling proteins, human UCP2 and UCP3, expressed in yeast, was performed to characterize fatty acid (FA)-induced H+ efflux in the resulted proteoliposomes. We now demonstrate for the first time that representatives of physiologically abundant long chain FAs, saturated or unsaturated, activate H+ translocation in UCP2- and UCP3-proteoliposomes. Efficiency of lauric, palmitic or linoleic acid was roughly the same, but oleic acid induced faster H+ uniport. We have confirmed that ATP and GTP inhibit such FA-induced H+ uniport mediated by UCP2 and UCP3. Coenzyme Q10 did not further significantly activate the observed H+ efflux. In conclusion, careful instant reconstitution yields intact functional recombinant proteins, UCP2 and UCP3, the activity of which is comparable with UCP1. |
| | |
Authors:
|
Markéta Záckova; Petr Jezek |
Related Documents
:
|
14754909 - Incorporation of cis-9,trans-11 or trans-10,cis-12 conjugated linoleic acid into plasma... 21569609 - Chemical stability study of vitamins thiamine, riboflavin, pyridoxine and ascorbic acid... 3236079 - Formation of a biotin precursor, pimelic acid, in yeasts from c18 fatty acids. 10923789 - Fatty acid hydroperoxide lyase in tomato fruits: cloning and properties of a recombinan... 2760909 - Linoleic acid supplementation, membrane lipids and leucocyte sodium transport in normot... 1151029 - Effect of electronic cooking on fatty acids in meats. 18614339 - Region-dependent absorption of faropenem shared with foscarnet, a phosphate transporter... 8593419 - Capillary gas chromatographic analysis of protein amino acids as their n(o,s)-isobutoxy... 9510849 - Simultaneous analysis of plasma free fatty acids and their 3-hydroxy analogs in fatty a... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Review |
Journal Detail:
|
Title: Bioscience reports Volume: 22 ISSN: 0144-8463 ISO Abbreviation: Biosci. Rep. Publication Date: 2002 Feb |
Date Detail:
|
Created Date: 2002-11-06 Completed Date: 2003-04-04 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8102797 Medline TA: Biosci Rep Country: United States |
Other Details:
|
Languages: eng Pagination: 33-46 Citation Subset: IM |
Affiliation:
|
Institute of Physiology, Academy of Sciences of the Czech Republic, Prague. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Carrier Proteins
/
metabolism Fatty Acids / metabolism Humans Ion Channels Membrane Transport Proteins* Mitochondria / metabolism* Mitochondrial Proteins* Proteins / metabolism* Yeasts / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Carrier Proteins; 0/Fatty Acids; 0/Ion Channels; 0/Membrane Transport Proteins; 0/Mitochondrial Proteins; 0/Proteins; 0/mitochondrial uncoupling protein 2; 0/mitochondrial uncoupling protein 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Thyroid hormones and mitochondria.
Next Document: Bcl-2 family of proteins: life-or-death switch in mitochondria.