| Reconstitution of import-competent outer membrane vesicles from mammalian mitochondria. | |
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MedLine Citation:
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PMID: 7798173 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Protein insertion into mitochondrial outer membrane (OM) vesicles isolated from Neurospora crassa has recently been reported. The N. crassa OM vesicles retained the features of the intact mitochondria concerning the dependency of insertion on the receptor protein [A. Mayer et al. (1993) J. Cell Biol. 121, 1233-1243]. In this study, OM vesicles were purified from bovine adrenal cortex mitochondria, and unilamellar proteoliposomes were reconstituted from OM vesicles using heptyl beta-thioglucoside. Both OM vesicles and the reconstituted outer membrane vesicles (ROM) were able to import porin, but unable to import the precursor of adrenodoxin, which translocates across both the outer and inner membranes of intact mitochondria. Porin insertion into both OM vesicles and ROM was inhibited in the presence of purified recombinant adrenodoxin precursor and also by ATP depletion, and was dependent on the trypsin-sensitive membrane surface factor, suggesting that the purified OM vesicles as well as ROM retained the properties of the intact OM concerning porin insertion. The protein import machinery of OM seems to be functional for the outer membrane protein without the participation of the inner membrane. The successful reconstitution of the protein import activity from solubilized OM will pave the way for further biochemical characterization of the protein import machinery of OM. |
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Authors:
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J Iwahashi; S Takaichi; K Mihara; T Omura |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biochemistry Volume: 116 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1994 Jul |
Date Detail:
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Created Date: 1995-01-25 Completed Date: 1995-01-25 Revised Date: 2007-12-19 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 156-63 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, Graduate School of Medical Science, Kyushu University. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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physiology Amino Acid Sequence Animals Biological Transport / physiology Cattle Intracellular Membranes / metabolism* Liposomes Membrane Proteins / metabolism* Mitochondria / metabolism*, ultrastructure Molecular Sequence Data Porins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Liposomes; 0/Membrane Proteins; 0/Porins; 56-65-5/Adenosine Triphosphate |
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