Document Detail

Reconstitution of dna synthetic capacity in senescent normal human fibroblasts by expressing cellular factors E2F and Mdm2.
MedLine Citation:
PMID:  11640890     Owner:  NLM     Status:  MEDLINE    
Unraveling the mechanisms underlying cellular senescence will contribute to the understanding of processes involved in aging and cancer. We sought to determine whether expression of cellular factors in senescent WI-38 human fibroblasts was sufficient to induce nuclear DNA synthesis. Expression by recombinant adenovirus of E2F1, E2F2, E2F3, cyclin E/cdk2, and Mdm2 individually resulted in DNA synthesis in 10-30% of cells. However, combination of Mdm2 with E2F or cyclin E/cdk2 resulted in 50 to 75% of cells synthesizing DNA. DNA synthesis occurred approximately 30 h following infection. We conclude that expression of normal cellular factors is sufficient to induce DNA synthesis in senescent normal human fibroblasts.
S Sarraj; R Farb; R E Martell
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  270     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-10-19     Completed Date:  2001-12-07     Revised Date:  2012-06-25    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  268-76     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Geriatric Research and Education Clinical Center, VA Medical Center, Durham, North Carolina 27705, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adenoviridae / genetics
CDC2-CDC28 Kinases*
Cell Aging / physiology*
Cell Cycle / physiology
Cell Cycle Proteins*
Cells, Cultured
Cyclin E / genetics
Cyclin-Dependent Kinase 2
Cyclin-Dependent Kinases / genetics
DNA-Binding Proteins*
E2F Transcription Factors
E2F1 Transcription Factor
E2F2 Transcription Factor
E2F3 Transcription Factor
Fibroblasts / cytology,  physiology*
Gene Expression / physiology
Nuclear Proteins*
Protein-Serine-Threonine Kinases / genetics
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins c-mdm2
Recombinant Proteins / genetics
Signal Transduction / physiology
Transcription Factors / genetics*
Grant Support
1K08AG/CA00915-01/AG/NIA NIH HHS; 2 P60 AG11268/AG/NIA NIH HHS
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Cyclin E; 0/DNA-Binding Proteins; 0/E2F Transcription Factors; 0/E2F1 Transcription Factor; 0/E2F1 protein, human; 0/E2F2 Transcription Factor; 0/E2F2 protein, human; 0/E2F3 Transcription Factor; 0/E2F3 protein, human; 0/Nuclear Proteins; 0/Proto-Oncogene Proteins; 0/Recombinant Proteins; 0/Transcription Factors; 9007-49-2/DNA; EC Kinases; EC Kinases; EC protein, human; EC Kinase 2; EC Kinases; EC protein, human; EC Proteins c-mdm2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Integrin- and cadherin-mediated induction of the matrix metalloprotease matrilysin in cocultures of ...
Next Document:  A multistep model for paclitaxel-induced apoptosis in human breast cancer cell lines.