| Reconstitution and characterization of NtrC protein in a deep-sea piezophilic bacterium, Shewanella violacea strain DSS12. | |
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MedLine Citation:
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PMID: 14519985 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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NtrC protein of piezophilic Shewanella violacea was overexpressed and purified, to confirm the protein-DNA interaction. An electrophoretic mobility shift assay demonstrated that the NtrC recognizes the sequence for NtrC binding within the region upstream of the glnA operon. Western blot analysis also showed that the NtrC is expressed at a higher level under high-pressure conditions than under atmospheric pressure conditions. |
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Authors:
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Hiroaki Kawano; Akihiko Ikegami; Kaoru Nakasone; Chiaki Kato; Ron Usami; Koki Horikoshi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 67 ISSN: 0916-8451 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2003 Sep |
Date Detail:
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Created Date: 2003-10-01 Completed Date: 2004-06-14 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 1983-5 Citation Subset: IM |
Affiliation:
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Department of Applied Chemistry, Faculty of Engineering, Toyo University, Kawagoe, Saitama, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics,
metabolism* Base Sequence Binding Sites DNA Probes / metabolism DNA-Binding Proteins / genetics, metabolism* Electrophoretic Mobility Shift Assay Escherichia coli / genetics, metabolism Escherichia coli Proteins Gene Expression Genes, Regulator Oceans and Seas Operon / genetics PII Nitrogen Regulatory Proteins Pressure Promoter Regions, Genetic Recombinant Fusion Proteins / genetics, metabolism Shewanella / genetics, metabolism* Trans-Activators / genetics, metabolism* Transcription Factors / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/DNA Probes; 0/DNA-Binding Proteins; 0/Escherichia coli Proteins; 0/PII Nitrogen Regulatory Proteins; 0/Recombinant Fusion Proteins; 0/Trans-Activators; 0/Transcription Factors; 0/glnG protein, E coli |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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