Document Detail

Reconstitution and characterization of NtrC protein in a deep-sea piezophilic bacterium, Shewanella violacea strain DSS12.
MedLine Citation:
PMID:  14519985     Owner:  NLM     Status:  MEDLINE    
NtrC protein of piezophilic Shewanella violacea was overexpressed and purified, to confirm the protein-DNA interaction. An electrophoretic mobility shift assay demonstrated that the NtrC recognizes the sequence for NtrC binding within the region upstream of the glnA operon. Western blot analysis also showed that the NtrC is expressed at a higher level under high-pressure conditions than under atmospheric pressure conditions.
Hiroaki Kawano; Akihiko Ikegami; Kaoru Nakasone; Chiaki Kato; Ron Usami; Koki Horikoshi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  67     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-10-01     Completed Date:  2004-06-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1983-5     Citation Subset:  IM    
Department of Applied Chemistry, Faculty of Engineering, Toyo University, Kawagoe, Saitama, Japan.
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MeSH Terms
Bacterial Proteins / genetics,  metabolism*
Base Sequence
Binding Sites
DNA Probes / metabolism
DNA-Binding Proteins / genetics,  metabolism*
Electrophoretic Mobility Shift Assay
Escherichia coli / genetics,  metabolism
Escherichia coli Proteins
Gene Expression
Genes, Regulator
Oceans and Seas
Operon / genetics
PII Nitrogen Regulatory Proteins
Promoter Regions, Genetic
Recombinant Fusion Proteins / genetics,  metabolism
Shewanella / genetics,  metabolism*
Trans-Activators / genetics,  metabolism*
Transcription Factors / genetics,  metabolism*
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA Probes; 0/DNA-Binding Proteins; 0/Escherichia coli Proteins; 0/PII Nitrogen Regulatory Proteins; 0/Recombinant Fusion Proteins; 0/Trans-Activators; 0/Transcription Factors; 0/glnG protein, E coli

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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