Document Detail


Reconstitution of the Catalytic Core of F-ATPase (αβ)(3) γ from Escherichia coli Using Chemically Synthesized Subunit γ
MedLine Citation:
PMID:  23212931     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Hooking up: Subunit γ of an F-ATPase with 11 point mutations was ligated chemically from six peptides and then reconstituted with further subunits of the (αβ)(3) γ catalytic core complex. The biologically active protein with 286 residues currently represents the longest nonredundant polypeptide chain synthesized by chemical means. The yields of the five consecutive ligations, especially the last three, dropped the total yield to only 0.005 %.
Authors:
Frank Wintermann; Siegfried Engelbrecht
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-4
Journal Detail:
Title:  Angewandte Chemie (International ed. in English)     Volume:  -     ISSN:  1521-3773     ISO Abbreviation:  Angew. Chem. Int. Ed. Engl.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370543     Medline TA:  Angew Chem Int Ed Engl     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Universität Osnabrück, Fachbereich Biologie/Chemie, Abt. Biochemie, Barbarastrasse 13, 49076 Osnabrück (Germany). frank.wintermann@biologie.uni-osnabrueck.de.
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