| A recommended laparoscopic procedure for implantation of microcapsules in the peritoneal cavity of non-human primates. | |
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MedLine Citation:
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PMID: 21435661 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The anatomical spatial distribution of microencapsulated islets transplanted into the peritoneal cavity of large animals remains a relatively unexplored area of study. In this study, we developed a new implantation approach using laparoscopy in order to avoid microcapsule amalgamation. This approach constitutes a clinically relevant method, which can be used to evaluate the distribution and in vivo biocompatibility of various types of transplanted microcapsules in the future. MATERIALS AND METHODS: Two healthy baboons were implanted intraperitoneally with microencapsulated islets through mini-laparotomy and observed at 76 d after implantation. Nine baboons underwent laparoscopic implantation of approximately 80,000 empty microcapsules. Microcapsule distribution was observed by laparoscopic camera during and after implantation at 1, 2, and 4 wk. At each time point, microcapsules were retrieved and evaluated with brightfield microscopy and histologic analysis. RESULTS: Mini-laparotomic implantation resulted in microcapusle aggregation in both baboons. In contrast, laparoscopic implantation resulted in even distribution of microcapsules throughout the peritoneum without sedimentation to the Douglas space in all animals. In eight out of nine animals, retrieved microcapsules were evenly distributed in the peritoneal cavity and presented with no pericapsular overgrowth and easily washed out during laparoscopic procedure. The one exception was attributed to microcapsule contamination with blood from the abdominal wall following trocar insertion. CONCLUSIONS: Laparoscopic implantation of microcapsules in non-human primates can be successfully performed and prevents microcapsule aggregation. Given the current widespread clinical application of laparoscopy, we propose that this presented laparoscopy technique could be applied in future clinical trials of microencapsulated islet transplantation. |
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Authors:
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Meirigeng Qi; Igor Lacik; Gabriela Kolláriková; Berit L Strand; Kjetil Formo; Yong Wang; Enza Marchese; Joshua E Mendoza-Elias; Katie P Kinzer; Francesca Gatti; Daniel Paushter; Sonny Patel; Jose Oberholzer |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-02-20 |
Journal Detail:
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Title: The Journal of surgical research Volume: 168 ISSN: 1095-8673 ISO Abbreviation: J. Surg. Res. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-05-02 Completed Date: 2011-07-07 Revised Date: 2013-05-23 |
Medline Journal Info:
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Nlm Unique ID: 0376340 Medline TA: J Surg Res Country: United States |
Other Details:
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Languages: eng Pagination: e117-23 Citation Subset: IM |
Copyright Information:
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Published by Elsevier Inc. |
Affiliation:
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Department of Surgery/Division of Transplantation, University of Illinois at Chicago, Illinois 60612, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Capsules* Female Islets of Langerhans Transplantation / methods* Laparoscopy / methods* Male Models, Animal Papio anubis Peritoneal Cavity / surgery* Time Factors Treatment Outcome |
| Grant Support | |
ID/Acronym/Agency:
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1 U42 RR023245-01/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Capsules |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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