Document Detail

Recombination in bacteriophage P2: recA dependent enhancement by ultraviolet irradiation and by transfection with mixed DNA dimers.
MedLine Citation:
PMID:  6929945     Owner:  NLM     Status:  MEDLINE    
Bacteriophage P2 is known for its exceptionally low rate of spontaneous (non-integrative) recombination, which however may be stimulated by ultraviolet irradiation of the phage. We show here that ligated dimers, made in vitro from mixtures of DNAs of two P2 mutants, upon transfection of lysozyme-spheroplasts give origin to recombinants at high frequency. While spontaneous P2 recombination occurs independently of the main recombination pathway of the bacteria, P2 recombinant formation following either ultraviolet irradiation or transfection with DNA dimers requires at least some element of such a pathway, since it is absent or greatly reduced in recA- bacteria or spheroplasts. It would seen that, in the course of its lytic development, P2 deploys a mechanism that inhibits the main recombination pathway of the host cell, or assumes DNA configurations refractory to it.
T Hudnik-Plevnik; G Bertani
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  178     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1980 Apr 
Date Detail:
Created Date:  1980-08-25     Completed Date:  1980-08-25     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  131-41     Citation Subset:  IM    
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MeSH Terms
Coliphages / genetics*
DNA, Viral / radiation effects
Pyrimidine Dimers / genetics*
Recombination, Genetic*
Ultraviolet Rays
Reg. No./Substance:
0/DNA, Viral; 0/Pyrimidine Dimers

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