Document Detail

Recombination of epithelial root sheath and dental papilla cells in vitro.
MedLine Citation:
PMID:  8987198     Owner:  NLM     Status:  MEDLINE    
Hertwig's epithelial root sheath (HERS) cells were isolated and recombined with ectomesenchymal cells in vitro utilizing extracellular matrix components as substrate. After 14 days in culture, HERS cells were differentiated and exhibited a stratified organization. These features resembled those observed in vivo as epithelial rests of Malassez. A mineralization process was also present in HERS cells, in which calcium salts were deposited. This mineralization was correlated with the strong immunoexpression of osteopontin by HERS. The results obtained add support to the possible role of HERS in the secretion of hypocalcified material on the root during early cementogenesis.
H Arzate; J Portilla Robertson; M E Aguilar Mendoza
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of medical research     Volume:  27     ISSN:  0188-4409     ISO Abbreviation:  Arch. Med. Res.     Publication Date:  1996  
Date Detail:
Created Date:  1997-01-30     Completed Date:  1997-01-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9312706     Medline TA:  Arch Med Res     Country:  MEXICO    
Other Details:
Languages:  eng     Pagination:  573-7     Citation Subset:  IM    
Laboratorio de Biología Celular y Molecular, Facultad de Odontología, Universidad Nacional Autónoma de México (UNAM), México, D.F.
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MeSH Terms
Calcium / analysis
Cell Differentiation / physiology
Cells, Cultured
Dental Cementum / cytology*,  metabolism
Dental Enamel / cytology
Dental Papilla / chemistry,  cytology*,  growth & development
Epithelial Cells
Epithelium / chemistry,  growth & development
Extracellular Matrix / metabolism
Mice, Inbred BALB C
Molar / cytology,  growth & development
Sialoglycoproteins / analysis
Tooth Calcification / physiology
Tooth Root / chemistry,  cytology*,  growth & development
Reg. No./Substance:
0/Sialoglycoproteins; 0/Spp1 protein, mouse; 106441-73-0/Osteopontin; 7440-70-2/Calcium

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