| Recombination-based telomere maintenance is dependent on Tel1-MRN and Rap1 and inhibited by telomerase, Taz1, and Ku in fission yeast. | |
| | |
MedLine Citation:
|
PMID: 18160711 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Fission yeast cells survive loss of the telomerase catalytic subunit Trt1 (TERT) through recombination-based telomere maintenance or through chromosome circularization. Although trt1Delta survivors with linear chromosomes can be obtained, they often spontaneously circularize their chromosomes. Therefore, it was difficult to establish genetic requirements for telomerase-independent telomere maintenance. In contrast, when the telomere-binding protein Taz1 is also deleted, taz1Delta trt1Delta cells are able to stably maintain telomeres. Thus, taz1Delta trt1Delta cells can serve as a valuable tool in understanding the regulation of telomerase-independent telomere maintenance. In this study, we show that the checkpoint kinase Tel1 (ATM) and the DNA repair complex Rad32-Rad50-Nbs1 (MRN) are required for telomere maintenance in taz1Delta trt1Delta cells. Surprisingly, Rap1 is also essential for telomere maintenance in taz1Delta trt1Delta cells, even though recruitment of Rap1 to telomeres depends on Taz1. Expression of catalytically inactive Trt1 can efficiently inhibit recombination-based telomere maintenance, but the inhibition requires both Est1 and Ku70. While Est1 is essential for recruitment of Trt1 to telomeres, Ku70 is dispensable. Thus, we conclude that Taz1, TERT-Est1, and Ku70-Ku80 prevent telomere recombination, whereas MRN-Tel1 and Rap1 promote recombination-based telomere maintenance. Evolutionarily conserved proteins in higher eukaryotic cells might similarly contribute to telomere recombination. |
| | |
Authors:
|
Lakxmi Subramanian; Bettina A Moser; Toru M Nakamura |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-12-26 |
Journal Detail:
|
Title: Molecular and cellular biology Volume: 28 ISSN: 1098-5549 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 2008 Mar |
Date Detail:
|
Created Date: 2008-02-15 Completed Date: 2008-03-17 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 1443-55 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, 900 S. Ashland Ave., MC669, Chicago, IL 60607, USA. nakamut@uic.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antigens, Nuclear
/
genetics DNA-Binding Proteins / antagonists & inhibitors*, genetics Plasmids Protein-Serine-Threonine Kinases / genetics, metabolism* Recombination, Genetic Schizosaccharomyces / genetics, physiology* Schizosaccharomyces pombe Proteins / antagonists & inhibitors*, genetics, metabolism* Telomerase / antagonists & inhibitors*, genetics Telomere / genetics, physiology* Telomere-Binding Proteins / antagonists & inhibitors*, genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
GM078253/GM/NIGMS NIH HHS; GM28039/GM/NIGMS NIH HHS; R01 GM078253-02/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, Nuclear; 0/DNA-Binding Proteins; 0/Ku autoantigen; 0/Rap1 protein, S pombe; 0/Schizosaccharomyces pombe Proteins; 0/Telomere-Binding Proteins; 0/taz1 protein, S pombe; EC 2.7.1.37/tel1 protein, S pombe; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.7.49/Telomerase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: ATP binding by monarch-1/NLRP12 is critical for its inhibitory function.
Next Document: Doubling the size of the glucocorticoid receptor ligand binding pocket by deacylcortivazol.