Document Detail

Recombination and assortment in the macronucleus of Tetrahymena thermophila: a theoretical study by computer simulation.
MedLine Citation:
PMID:  6510707     Owner:  NLM     Status:  MEDLINE    
The compound nature of the macronucleus of Tetrahymena thermophila presents multiple opportunities for recombination between genes on the same macronuclear chromosome. Such recombinants should be detectable through their assortment at subsequent amitotic macronuclear divisions. Thus, a macronucleus that is initially AB/ab should produce recombinant assortees of the genotypes Ab/aB. Computer simulation shows that, when the recombination frequency is two or fewer times per cell cycle, recombinant assortees are produced at experimentally measurable frequencies of less than 40%. At higher recombination frequencies, linked genes appear to assort independently. The simulations also show that recombination during macronuclear development can be distinguished from recombination in subsequent cell cycles only if the first appearance of recombinant assortees is 100 or more fissions after conjugation. The use of macronuclear recombination and assortment as a means of mapping macronuclear genes is severely constrained by the large variances in assortment outcomes; with experimentally small sample sizes, such mapping is impossible.
F P Doerder; S L Diblasi
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  108     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  1984 Dec 
Date Detail:
Created Date:  1985-02-05     Completed Date:  1985-02-05     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1035-45     Citation Subset:  IM    
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MeSH Terms
Cell Nucleus / physiology
Crossing Over, Genetic
Models, Genetic*
Recombination, Genetic*
Tetrahymena / genetics*,  physiology
Grant Support

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