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Recombination adenovirus-mediated human lactoferrin cDNA inhibits the growth of human MCF-7 breast cancer cells.
MedLine Citation:
PMID:  22309278     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Objectives  Human lactoferrin, an 80 kDa iron-binding glycoprotein, has antitumour effects. We have explored the potential therapeutic role of re-expressing human lactoferrin gene product in human breast cancer. Methods  A recombinant adenovirus expressing the human lactoferrin cDNA (ad-hLTF) was constructed and used to infect breast cancer cells. Key findings  Seventy-two hours after infection, ad-hLTF had considerable cytotoxicity on MCF-7 cells. A time-course study showed that ad-hLTF infection of MCF-7 cells at 100 plaque-forming units per cell increased the number of cells in G(0) /G(1) phase and appeared markedly at Sub-G(1) apoptotic peak. The presence of apoptotic cells was confirmed using Annexin V-fluoresecein isothiocyanate apoptosis detection by flow cytometry. Ad-hLTF also resulted in a decrease of Bcl-2 protein and an increase in Bax protein. Conclusions  Ad-hLTF plays an important role in the induction of cell cycle arrest and apoptosis in MCF-7 cells. The results demonstrated that ad-hLTF could have potential benefits in the treatment of breast cancer.
Authors:
Jianjie Wang; Qingwang Li; Yetao Ou; Kun Li; Zengsheng Han; Peijun Wang; Shaobo Zhou
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Publication Detail:
Type:  Journal Article     Date:  2011-11-30
Journal Detail:
Title:  The Journal of pharmacy and pharmacology     Volume:  64     ISSN:  2042-7158     ISO Abbreviation:  J. Pharm. Pharmacol.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376363     Medline TA:  J Pharm Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  457-63     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors. JPP © 2011 Royal Pharmaceutical Society.
Affiliation:
Department of Biological Engineering, College of Environment and Chemical Engineering, Yanshan University, Qinhuangdao School of Animal Science, Northwest A&F University, Yangling College of Basic Medicine, Jiamusi University, Jiamusi, China Institute of Research in the Applied Natural Sciences, University of Bedfordshire, Luton, UK.
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