Document Detail

Recombination adenovirus-mediated human lactoferrin cDNA inhibits the growth of human MCF-7 breast cancer cells.
MedLine Citation:
PMID:  22309278     Owner:  NLM     Status:  In-Data-Review    
Objectives  Human lactoferrin, an 80 kDa iron-binding glycoprotein, has antitumour effects. We have explored the potential therapeutic role of re-expressing human lactoferrin gene product in human breast cancer. Methods  A recombinant adenovirus expressing the human lactoferrin cDNA (ad-hLTF) was constructed and used to infect breast cancer cells. Key findings  Seventy-two hours after infection, ad-hLTF had considerable cytotoxicity on MCF-7 cells. A time-course study showed that ad-hLTF infection of MCF-7 cells at 100 plaque-forming units per cell increased the number of cells in G(0) /G(1) phase and appeared markedly at Sub-G(1) apoptotic peak. The presence of apoptotic cells was confirmed using Annexin V-fluoresecein isothiocyanate apoptosis detection by flow cytometry. Ad-hLTF also resulted in a decrease of Bcl-2 protein and an increase in Bax protein. Conclusions  Ad-hLTF plays an important role in the induction of cell cycle arrest and apoptosis in MCF-7 cells. The results demonstrated that ad-hLTF could have potential benefits in the treatment of breast cancer.
Jianjie Wang; Qingwang Li; Yetao Ou; Kun Li; Zengsheng Han; Peijun Wang; Shaobo Zhou
Related Documents :
15268778 - Synchronization of goat fibroblast cells at quiescent stage and determination of their ...
362178 - Cell-cycle variation in the induction of lethality and mitotic recombination after trea...
9732748 - A temperature-sensitive dna synthesis mutant isolated from the chinese hamster ovary ce...
2715188 - Control of cell division in hepatoma cells by exogenous heparan sulfate proteoglycan.
15566568 - The establishment and characterization of the first canine hepatocellular carcinoma cel...
7391538 - Production and reproductive performance of hexaparental and octaparental mice.
Publication Detail:
Type:  Journal Article     Date:  2011-11-30
Journal Detail:
Title:  The Journal of pharmacy and pharmacology     Volume:  64     ISSN:  2042-7158     ISO Abbreviation:  J. Pharm. Pharmacol.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376363     Medline TA:  J Pharm Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  457-63     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors. JPP © 2011 Royal Pharmaceutical Society.
Department of Biological Engineering, College of Environment and Chemical Engineering, Yanshan University, Qinhuangdao School of Animal Science, Northwest A&F University, Yangling College of Basic Medicine, Jiamusi University, Jiamusi, China Institute of Research in the Applied Natural Sciences, University of Bedfordshire, Luton, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Primary human sinonasal epithelial cell culture model for topical drug delivery in patients with chr...
Next Document:  Ir-Catalyzed Allylic Alkylation Reaction with N-Aryl Phosphoramidite Ligands: Scope and Mechanistic ...