Document Detail


Recombination activator function of the novel RAD51- and RAD51B-binding protein, human EVL.
MedLine Citation:
PMID:  19329439     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The RAD51 protein is a central player in homologous recombinational repair. The RAD51B protein is one of five RAD51 paralogs that function in the homologous recombinational repair pathway in higher eukaryotes. In the present study, we found that the human EVL (Ena/Vasp-like) protein, which is suggested to be involved in actin-remodeling processes, unexpectedly binds to the RAD51 and RAD51B proteins and stimulates the RAD51-mediated homologous pairing and strand exchange. The EVL knockdown cells impaired RAD51 assembly onto damaged DNA after ionizing radiation or mitomycin C treatment. The EVL protein alone promotes single-stranded DNA annealing, and the recombination activities of the EVL protein are further enhanced by the RAD51B protein. The expression of the EVL protein is not ubiquitous, but it is significantly expressed in breast cancer-derived MCF7 cells. These results suggest that the EVL protein is a novel recombination factor that may be required for repairing specific DNA lesions, and that may cause tumor malignancy by its inappropriate expression.
Authors:
Motoki Takaku; Shinichi Machida; Noriko Hosoya; Shugo Nakayama; Yoshimasa Takizawa; Isao Sakane; Takehiko Shibata; Kiyoshi Miyagawa; Hitoshi Kurumizaka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-26
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  284     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-18     Completed Date:  2009-07-07     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  14326-36     Citation Subset:  IM    
Affiliation:
Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, and Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan.
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MeSH Terms
Descriptor/Qualifier:
Cell Adhesion Molecules / metabolism*
Cell Line, Tumor
Chromosome Pairing
DNA / metabolism
DNA-Binding Proteins / metabolism*
Gene Knockdown Techniques
Humans
Protein Binding
Rad51 Recombinase / metabolism*
Recombination, Genetic*
Chemical
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/DNA-Binding Proteins; 0/EVL protein, human; 0/RAD51B protein, human; 9007-49-2/DNA; EC 2.7.7.-/Rad51 Recombinase
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