Document Detail


Recombinant strains of Escherichia coli for L-aspartic acid biosynthesis.
MedLine Citation:
PMID:  17650676     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aspartase overproducing mutant B-715 was used as a donor of the aspartase gene for further construction of the aspartase-hyperproducing strains by molecular cloning. In preliminary experiments activity of transformants and their efficiency in L-aspartic acid biosynthesis were compared. The conditions for recombinant strain multiplication, biomass activation and L-aspartic acid biosynthesis were optimized. The optimum temperature for cells multiplication, their activation and for product biosynthesis was 37 degrees C. Two-stage process of the multiplication of bacteria (first in LB medium, and then in FF medium) eliminates the appearing of the inclusion bodies of aspartase in the cells. The shaking during cell activation improved cells productivity. The change of pH in the course of the biosynthesis process was insignificant but did not influence the process.
Authors:
Grazyna Gadomska; Andrzej Płucienniczak; Aleksander Chmiel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Polish journal of microbiology / Polskie Towarzystwo Mikrobiologów = The Polish Society of Microbiologists     Volume:  56     ISSN:  1733-1331     ISO Abbreviation:  Pol. J. Microbiol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-07-26     Completed Date:  2007-08-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101229003     Medline TA:  Pol J Microbiol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  77-82     Citation Subset:  IM    
Affiliation:
Department of Biosynthesis of Drugs, Medical University of Lódź, Lódź, Poland.
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MeSH Terms
Descriptor/Qualifier:
Aspartate Ammonia-Lyase / genetics*
Aspartic Acid / biosynthesis*
Biomass
Cloning, Molecular
Escherichia coli / enzymology,  genetics*,  growth & development,  metabolism*
Hydrogen-Ion Concentration
Recombinant Proteins / genetics
Temperature
Transformation, Bacterial
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 56-84-8/Aspartic Acid; EC 4.3.1.1/Aspartate Ammonia-Lyase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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