| Recombinant rat IL-1beta and IL-6 synergistically enhance C3 mRNA levels and complement component C3 secretion by H-35 rat hepatoma cells. | |
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MedLine Citation:
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PMID: 15804599 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hepatic synthesis of complement component C3 is regulated in part by inflammatory cytokines. Rat models are frequently employed to investigate pathogenic roles of complement and cytokines. However, cytokines obtained from species other than the rat were used in previous studies of cytokine regulation of C3 synthesis in rat hepatocytes or hepatoma cells. It is not known whether these prior reports predict hepatocellular responses evoked by rat cytokines. Therefore, H-35 rat hepatoma cells were employed to measure the effect of recombinant rat IL-1beta, IL-6, IFN-gamma, and TNF-alpha on C3 protein secretion and C3 mRNA levels quantified by ELISA and quantitative RT-PCR. Compared to untreated control cells, H-35 cells treated with IL-1beta, IL-6, and IFN-gamma increased C3 secretion approximately 10-, 4-, and 2-fold, respectively. TNF-alpha was toxic, precluding further analysis. IL-1beta and IL-6 demonstrated synergy with respect to the quantity and rate of increase of C3 mRNA measured and the magnitude of C3 protein secretion. Previous reports using non-rat cytokines did not consistently predict H-35 responses to rat cytokines. Consequently, we recommend the use of rat cytokines in rat models that include analysis of cytokine-mediated events. |
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Authors:
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Joan M Stapp; Virginie Sjoelund; Herbert A Lassiter; Richard C Feldhoff; Pamela W Feldhoff |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cytokine Volume: 30 ISSN: 1043-4666 ISO Abbreviation: Cytokine Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-04-04 Completed Date: 2005-07-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9005353 Medline TA: Cytokine Country: United States |
Other Details:
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Languages: eng Pagination: 78-85 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, Division of Neonatal Medicine, University of Louisville School of Medicine, Louisville, KY 40202, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Complement C3 / drug effects, genetics*, secretion* Dose-Response Relationship, Drug Drug Synergism Interleukin-1 / genetics, pharmacology* Interleukin-6 / genetics, pharmacology* Liver Neoplasms, Experimental / drug therapy, genetics, immunology* Protein Precursors / drug effects, genetics*, secretion* RNA, Messenger / drug effects Rats Recombinant Proteins / pharmacology Tumor Necrosis Factor-alpha / pharmacology Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Complement C3; 0/Interleukin-1; 0/Interleukin-6; 0/Protein Precursors; 0/RNA, Messenger; 0/Recombinant Proteins; 0/Tumor Necrosis Factor-alpha |
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