Document Detail

Recombinant production of hyperthermostable CelB from Pyrococcus furiosus in Lactobacillus sp.
MedLine Citation:
PMID:  22714098     Owner:  NLM     Status:  Publisher    
Lactic acid bacteria (LAB) are used widespread in the food industry as traditional starters for various fermented foods. For recombinant protein production, LAB would be superior with view from the food safety demands since most of them are Generally Recognized As Safe organisms. We investigated the two pSIP expression systems, pSIP403 and pSIP409 (Sørvig et al. 2005), to produce a hyper-thermophilic β-glycosidase (CelB) from Pyrococcus furiosus in Lactobacillus plantarum NC8 and Lactobacillus casei as hosts, respectively. Both lactobacilli harboring the pSIP409-celB vector produced active CelB in batch bioreactor cultivations (MRS medium) while the specific CelB activity of the cell free extract was about 44 % higher with L. plantarum (1,590 ± 90 nkat/mg(protein)) than with L. casei (1,070 ± 66 nkat/mg(protein)) using p-nitrophenyl-β-galactoside (pNPGal) as the substrate. A fed-batch bioreactor cultivation of L. plantarum NC8 pSIP409-celB resulted in a specific CelB activity of 2,500 ± 120 nkat ( pNPGal)/mg(protein) after 28 h. A repeated dosage of the inducer spp-IP did not increase the enzyme expression further. As alternative for the cost intensive MRS medium, a basal whey medium with supplements (yeast extract, Tween 80, NH(4)-citrate) was developed. In bioreactor cultivations using this medium, about 556 ± 29 nkat ( pNPGal)/mg(protein) of CelB activity was achieved. It was shown that both LAB were potential expression hosts for recombinant enzyme production. The pSIP expression system can be applied in L. casei.
N Böhmer; S Lutz-Wahl; L Fischer
Related Documents :
20598448 - Prospects for new technology of meat processing in japan.
16233408 - Chitosan productivity enhancement in rhizopus oryzae ypf-61a by d-psicose.
21593288 - Review: starch matrices and the glycemic response.
521628 - Developing new food products.
24692268 - Livestock and food security: vulnerability to population growth and climate change.
17553548 - Sex differences noted in mercury bioaccumulation in magicicada cassini.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-20
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  -     ISSN:  1432-0614     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Institute of Food Science and Biotechnology, Department of Biotechnology, University of Hohenheim, Garbenstr. 25, 70599, Stuttgart, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Enhancement of recombinant human EPO production and glycosylation in serum-free suspension culture o...
Next Document:  Relationship between Serum Omentin-1 Level and Bone Mineral Density in Girls with Anorexia Nervosa.