Document Detail

MedLine Citation:
PMID:  11421077     Owner:  NLM     Status:  MEDLINE    
Previously, two pools of phytochrome A (phyA' and phyA") have been detected by in situ low-temperature fluorescence spectroscopy and photochemistry; it was suggested that they might differ in the nature of their posttranslational modification. In order to verify this possibility Arabidopsis and rice (Oryza) phyA were expressed in yeast and the pigments were assembled in vivo with phycocyanobilin (PCB) and phytochromobilin (P phi B). The resulting recombinant phytochromes in the red-light-absorbing form (Pr) were characterized in the yeast cell by (1) the fluorescence emission spectra; (2) the temperature dependence of Pr fluorescence intensity and activation energy of fluorescence decay; and (3) the extent of photoconversion of Pr into photoproduct lumi-R (gamma 1) or far-red-light absorbing form (Pfr) (gamma 2). Both Arabidopsis phyA/PCB and Oryza phyA/P phi B had low gamma 1 of ca 0.05, allowing their attribution to the Pr" phenomenological type of phytochrome comprising phyA", phyB and cryptogam phytochromes. The spectroscopic properties of Oryza phyA/P phi B were also very close to phyA". However, both investigated holoproteins differed from phyA", both with respect to the character of temperature dependence of the fluorescence yield and activation energy. Thus, recombinant Oryza phyA/P phi B is similar but not identical to phyA". The data demonstrate that the low-abundance-fraction plant phyA (phyA") comes from the same gene as the major (phyA') fraction. Because both endogenous phyA fractions differ from the phytochrome expressed in yeast, they appear to be posttranslationally modified and/or bound to partner proteins or cellular substructures. However, the character of the presumed chemical modification is different in phyA' and phyA" and its extent is more profound in the case of the former.
V Sineshchekov; L Hennig; T Lamparter; J Hughes; W Gärtner; E Schäfer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  73     ISSN:  0031-8655     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-06-25     Completed Date:  2001-07-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  692-6     Citation Subset:  IM    
Department of Biology, M. V. Lomonosov Moscow State University, 119899 Moscow, Russian Federation.
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MeSH Terms
Arabidopsis Proteins
Phytochrome / chemistry*,  genetics,  radiation effects
Phytochrome A
Protein Processing, Post-Translational
Recombinant Proteins / chemistry,  genetics,  radiation effects
Saccharomyces cerevisiae / genetics
Spectrometry, Fluorescence
Reg. No./Substance:
0/Arabidopsis Proteins; 0/PHYA protein, Arabidopsis; 0/Phytochrome A; 0/Recombinant Proteins; 11121-56-5/Phytochrome

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