Document Detail


Recombinant interleukin-4 enhances Campylobacter jejuni invasion of intestinal pig epithelial cells (IPEC-1).
MedLine Citation:
PMID:  19409975     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Campylobacter jejuni, a leading cause of bacterial gastroenteritis, has a diverse spectrum of disease expression. Polymicrobial infections may contribute to this, such as Trichuris, which elicits type 2 cytokines (including IL-4) and downregulates type 1 immunity. In previous studies, gnotobiotic piglets infected with C. jejuni and Trichuris suis had bloody diarrhea and marked gastrointestinal pathology, including bacterial invasion into epithelial cells and macrophages. Neonatal swine given these dual infections had elevated IL-4 and IL-10 responses in feces. In the studies reported here, we hypothesized that IL-4 or IL-10 enhances invasion of intestinal pig epithelial cells (IPEC-1) by C. jejuni. 10-14-day-old IPEC-1 cells were pretreated with recombinant IL-4 (rIL-4) or rIL-10 for 5h and then challenged with C. jejuni. Cells pretreated with rIL-4 were viable and showed approximately 6-fold increases in C. jejuni (but not Escherichia coli DH5alpha) internalization compared to cells with no pretreatment. Enhanced C. jejuni invasion was rIL-4 dose-dependent and reversed by addition of anti-IL-4 antibody. Preincubation with rIL-10 did not significantly alter C. jejuni internalization. Transepithelial electrical resistance (TEER) was significantly reduced following rIL-4 treatment, but not rIL-10 treatment. After rIL-4 pretreatment and C. jejuni challenge, light microscopy showed vacuolated cells with damaged paracellular junctions. Transmission electron microscopy (TEM) showed multiple internalized bacteria. Most were in the cytoplasm, but some were within or adjacent to vacuoles. We conclude that rIL-4 damages paracellular junctions and alters the physiology of these epithelial cells allowing increased invasion of C. jejuni.
Authors:
G Parthasarathy; L S Mansfield
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-05-03
Journal Detail:
Title:  Microbial pathogenesis     Volume:  47     ISSN:  1096-1208     ISO Abbreviation:  Microb. Pathog.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-23     Completed Date:  2009-08-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8606191     Medline TA:  Microb Pathog     Country:  England    
Other Details:
Languages:  eng     Pagination:  38-46     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Molecular Genetics, Comparative Enteric Diseases Laboratory, National Food Safety and Toxicology Center, Michigan State University, East Lansing, MI 48824, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Campylobacter jejuni / immunology*,  pathogenicity*
Cell Membrane / ultrastructure
Cells, Cultured
Cytoplasm / microbiology,  ultrastructure
Epithelial Cells / microbiology*,  ultrastructure
Escherichia coli / immunology,  pathogenicity
Interleukin-10 / immunology
Interleukin-4 / immunology*
Intestinal Mucosa / cytology
Microscopy
Microscopy, Electron, Transmission
Recombinant Proteins / immunology
Swine
Grant Support
ID/Acronym/Agency:
AI42348-01/AI/NIAID NIH HHS; N01-AI-30058/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 130068-27-8/Interleukin-10; 207137-56-2/Interleukin-4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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