| Recombinant human müllerian inhibiting substance inhibits epidermal growth factor receptor tyrosine kinase. | |
| | |
MedLine Citation:
|
PMID: 2560399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Autophosphorylation of the epidermal growth factor (EGF) receptor in A-431 cells and plasma membrane fractions was inhibited by partially purified recombinant human Müllerian Inhibiting Substance (MIS). Immunoprecipitation of the EFG receptor using anti-EGF receptor or anti-phosphotyrosine antibodies, and phosphoamino acid analysis of this receptor, demonstrated that MIS specifically inhibited EGF-induced tyrosine phosphorylation. Inhibition of EGF receptor autophosphorylation by MIS in membrane preparations was not affected by increasing concentrations of EGF, manganese or [gamma-(32)P] ATP. Thus, it is unlikely that MIS competes for EGF binding sites or sequesters substrate. Immunoabsorption of MIS with anti-human MIS antibody blocked the MIS inhibition of EGF receptor autophosphorylation, indicating that the inhibition was due to MIS. Our data suggest that MIS regulates the activity of the EGF receptor tyrosine kinase in A-431 cells. |
| | |
Authors:
|
F G Cigarroa; J P Coughlin; P K Donahoe; M F White; N Uitvlugt; D T MacLaughlin |
Related Documents
:
|
14615869 - Ganglioside-liposome immunoassay for the detection of botulinum toxin. 2983339 - Stimulation of glucose uptake by transforming growth factor beta: evidence for the requ... 11145289 - Increased expression of epidermal growth factor-receptor in an experimental model of co... 3260329 - Chicken epidermal growth factor (egf) receptor: cdna cloning, expression in mouse cells... 6273899 - Rotational diffusion of epidermal growth factor complexed to cell surface receptors ref... 16267289 - Stabilization of polar localization of a chemoreceptor via its covalent modifications a... 3038579 - Diprenorphine has agonist activity at opioid kappa-receptors in the myenteric plexus of... 2061919 - Dexamethasone 21-(beta-isothiocyanatoethyl) thioether: a new affinity label for glucoco... 19389919 - Trace amine-associated receptors as emerging therapeutic targets. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Growth factors (Chur, Switzerland) Volume: 1 ISSN: 0897-7194 ISO Abbreviation: Growth Factors Publication Date: 1989 |
Date Detail:
|
Created Date: 1990-04-11 Completed Date: 1990-04-11 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 9000468 Medline TA: Growth Factors Country: SWITZERLAND |
Other Details:
|
Languages: eng Pagination: 179-91 Citation Subset: IM |
Affiliation:
|
Pediatric Surgical Research Laboratory, Massachusetts General Hospital, Harvard Medical School, Boston 02114. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
analysis Animals Anti-Mullerian Hormone Cell Membrane / metabolism Cells, Cultured Female Glycoproteins* Growth Inhibitors / physiology* Humans Mullerian Ducts / cytology Organ Culture Techniques Phosphorylation Protein-Tyrosine Kinases / antagonists & inhibitors* Rats Receptor, Epidermal Growth Factor / drug effects, metabolism* Recombinant Proteins / pharmacology Testicular Hormones / physiology* |
| Grant Support | |
ID/Acronym/Agency:
|
17393//PHS HHS |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Glycoproteins; 0/Growth Inhibitors; 0/Recombinant Proteins; 0/Testicular Hormones; 80497-65-0/Anti-Mullerian Hormone; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.1/Receptor, Epidermal Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: IgG specifically enhances chrysotile asbestos-stimulated superoxide anion production by the alveolar...
Next Document: Aneurysmectomy prognosticators by equilibrium multi-gated cardiac blood pool scintigraphy.