Document Detail


Recombinant human collagen III gel for transplantation of autologous skin cells in porcine full-thickness wounds.
MedLine Citation:
PMID:  23362205     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Complex skin wounds, such as chronic ulcers and deep burns, require lengthy treatments and cause extensive burdens on healthcare and the economy. Use of biomaterials and cell transplantation may improve traditional treatments and promote the healing of difficult-to-treat wounds. In this study, we investigated the use of recombinant human collagen III (rhCol-III) gel as a delivery vehicle for cultured autologous skin cells (keratinocytes only or keratinocyte-fibroblast mixtures). We examined its effect on the healing of full-thickness wounds in a porcine wound-healing model. Two Landrace pigs were used for the study. Fourteen deep dermal wounds were created on the back of each pig with an 8 mm biopsy punch. Syringes containing acellular rhCol-III gel (n = 8) or rhCol-III gel with autologous keratinocytes (n = 8) or rhCol-III gel with autologous keratinocytes and fibroblasts (n = 8) were applied into wounds. Untreated wounds were used as controls for the treatment groups (n = 4). We used rhCol-III gel to manufacture a cell-delivery syringe containing autologous skin cells. In a full-thickness wound-healing model, we observed that rhCol-III gel enhances early granulation tissue formation. Interestingly, we found cell type-dependent differences in the stability of rhCol-III in vivo. Fibroblast-containing gel was effectively removed from the wound, whereas gels without cells or with keratinocytes only remained intact. Our results demonstrate that the properties of rhCol-III gel for skin cell transplantation can be significantly altered in a cell type-dependent manner. Copyright © 2013 John Wiley & Sons, Ltd.
Authors:
Kristo Nuutila; Matti Peura; Sari Suomela; Mika Hukkanen; Antti Siltanen; Ari Harjula; Jyrki Vuola; Esko Kankuri
Related Documents :
6090365 - Combined effect of diethyldithiocarbamate (ddc) and modest hyperthermia on chinese hams...
12476615 - The effect of cisplatin, etoposide and quercetin on hsp72 expression.
9843825 - Specific heat shock proteins protect microtubules during simulated ischemia in cardiac ...
12439755 - Induction of inducible nitric oxide synthase by epstein-barr virus b95-8-derived lmp1 i...
21171515 - Lipoblast-like signet-ring cells in neurofibroma: a potential diagnostic pitfall of mal...
22896795 - Evolution of the discs large gene family provides new insights into the establishment o...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-30
Journal Detail:
Title:  Journal of tissue engineering and regenerative medicine     Volume:  -     ISSN:  1932-7005     ISO Abbreviation:  J Tissue Eng Regen Med     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101308490     Medline TA:  J Tissue Eng Regen Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 John Wiley & Sons, Ltd.
Affiliation:
Institute of Biomedicine, Pharmacology, University of Helsinki, Finland; 3rd Department of Surgery, Helsinki University Central Hospital, Finland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Hypoxia Increases the Yield of Photoreceptors Differentiating from Mouse Embryonic Stem Cells and Im...
Next Document:  Multifarious roles of intrinsic disorder in proteins illustrate its broad impact on plant biology.